PXD031311-1

PXD031311 is an original dataset announced via ProteomeXchange.

Title	RIF1-ASF1-mediated high-order chromatin structure safeguards genome integrity
Description	The 53BP1-RIF1 pathway antagonizes resection of DNA broken ends and confers PARP inhibitor sensitivity on BRCA1-mutated tumors. However, it is unclear how this pathway suppresses initiation of resection. Here, we identify ASF1 as a partner of RIF1 via an interacting manner similar to its interactions with histone chaperones CAF-1 and HIRA. ASF1 is recruited to distal chromatin flanking DNA breaks by 53BP1-RIF1 and promotes non-homologous end joining (NHEJ) using its histone chaperone activity. Epistasis analysis shows that ASF1 acts in the same NHEJ pathway as RIF1, but via a parallel pathway with the shieldin complex, which suppresses resection after initiation. Moreover, defects in end resection and homologous recombination (HR) in BRCA1-deficient cells are largely suppressed by ASF1 deficiency. Mechanistically, ASF1 compacts adjacent chromatin by heterochromatinization to protect broken DNA ends from BRCA1-mediated resection. Taken together, our findings identified a RIF1-ASF1 histone chaperone complex that promotes changes in high-order chromatin structure to stimulate the NHEJ pathway for DSB repair.
HostingRepository	PRIDE
AnnounceDate	2022-02-04
AnnouncementXML	Submission_2022-02-04_01:19:30.639.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	kejiao Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-30 10:35:36	ID requested
⏵ 1	2022-02-04 01:19:30	announced

Dataset with its publication pending

submitter keyword: human,HEK293T,LC-MS

Dongyi. Xu
contact affiliation	State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China 100871.
contact email	xudongyi@pku.edu.cn
lab head
kejiao Li
contact affiliation	Peking University
contact email	1801110526@pku.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD031311

PRIDE project URI

• PRIDE
  1. PXD031311
     1. Label: PRIDE project
     2. Name: RIF1-ASF1-mediated high-order chromatin structure safeguards genome integrity