PXD031267-2

PXD031267 is an original dataset announced via ProteomeXchange.

Title	A repurposed, non-canonical cytochrome c, chaperones calcium binding by PilY1 for type IVa pili formation - Dataset 2/2
Description	Type IVa pili (T4aP) are versatile bacterial cell surface structures that undergo extension/adhesion/retraction cycles powered by the cell envelope-spanning T4aP machine. In this machine, a complex composed of four minor pilins and PilY1 primes T4aP extension and is also present at the pilus tip mediating adhesion. Similar to many other bacteria, Myxococcus xanthus contains multiple minor pilins/PilY1 sets, the function of which remains unknown.Here, we report that minor pilins and PilY1 of cluster_1 (PilY1.1) form calcium-responsive priming and tip complexes contingent on a non-canonical cytochrome c (TfcP) with an unusual His/Cys heme ligation. We provide evidence that TfcP is unlikely to participate in electron transport and has been repurposed to promote calcium binding by PilY1.1 at low calcium concentrations, thereby stabilising PilY1.1 and enabling its function in a broader range of calcium concentrations. These results identify a novel function of cytochromes c and illustrate how incorporating an accessory factor expands the environmental range under which the T4aP system functions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:31:02.775.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Timo Glatter
SpeciesList	scientific name: Myxococcus xanthus DK 1622; NCBI TaxID: 246197;
ModificationList	No PTMs are included in the dataset
Instrument	Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-26 06:35:31	ID requested
1	2022-01-30 13:36:48	announced
⏵ 2	2024-10-22 05:31:03	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Myxococcus xanthus

Timo Glatter
contact affiliation	Max Planck Institute for Terrestrial Microbiology 35043 Marburg Germany
contact email	glatter@mpi-marburg.mpg.de
lab head
Timo Glatter
contact affiliation	MPI Marburg
contact email	glatter@mpi-marburg.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD031267

PRIDE project URI

• PRIDE
  1. PXD031267
     1. Label: PRIDE project
     2. Name: A repurposed, non-canonical cytochrome c, chaperones calcium binding by PilY1 for type IVa pili formation - Dataset 2/2