PXD031231-1

PXD031231 is an original dataset announced via ProteomeXchange.

Title	Intestine proteome of Rhipicephalus microplus tick reveal key molecular features associated with resistant to ivermectin
Description	Rhipicephalus microplus is among the critical tick parasite of cattle farming globally. However, the massive use of ivermectin last decades induced the generation of the tick-resistant population. Unfortunately, despite extensive research the mode of action of ivermectin remains unclear. Due to the limited molecular information related to the resistance of R. microplus to ivermectin, we carried out a midgut comparative proteomics study between susceptible and resistant tick. Our tissues specificity approach also included tandem mass tags (TMT) couple to synchronous precursor selection (SPS)-MS3. Our proteomics scrutiny exhibits key biological process associated with blood digestion and anticoagulation in midgut. In addition, we could observe the significant shutdown of the translation mechanism in susceptible thinks. At the same time, resistant thick exhibited the over-accumulation of cytochrome P450, glutathione-S-transferase, and ABC transporters. A similar trend was observed in proteins like glutathione peroxides glutaredoxin, sulfiredoxin, and disulfide-isomerase, which suggest a possible activation of the oxidative metabolism as a potential strategy to cope with ivermectin treatment.
HostingRepository	PRIDE
AnnounceDate	2024-01-29
AnnouncementXML	Submission_2024-01-29_15:06:05.673.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Miguel Elizalde
SpeciesList	scientific name: Rhipicephalus microplus; NCBI TaxID: 6941;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-25 00:58:42	ID requested
⏵ 1	2024-01-29 15:06:06	announced

Ruiz-May E, Á, lvarez-S, á, nchez ME, Aguilar-Tipacam, ú G, Elizalde-Contreras JM, Boj, ó, rquez-Vel, á, zquez E, Zamora-Brise, ñ, o JA, V, á, zquez-Carrillo LI, L, ó, pez-Esparza A, Comparative proteome analysis of the midgut of Rhipicephalus microplus (Acari: Ixodidae) strains with contrasting resistance to ivermectin reveals the activation of proteins involved in the detoxification metabolism. J Proteomics, 263():104618(2022) [pubmed]

10.1016/j.jprot.2022.104618;

submitter keyword: LC-MS, ivermectin, SPS, proteome, Rhipicephalus microplus

Ruiz-May Eliel
contact affiliation	Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C., Cluster BioMimic®, Carretera Antigua a Coatepec 351, El Haya, Xalapa, Veracruz, Mexico; C.P. 91073
contact email	eliel.ruiz@inecol.mx
lab head
Miguel Elizalde
contact affiliation	Instituto de Ecolog'ia A.C.
contact email	jose.elizalde@inecol.mx
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD031231

PRIDE project URI

• PRIDE
  1. PXD031231
     1. Label: PRIDE project
     2. Name: Intestine proteome of Rhipicephalus microplus tick reveal key molecular features associated with resistant to ivermectin