PXD031228 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Vascular bed systematic profiling unravels endothelial and glycocalyx dysregulations in experimental high salt hypertension |
| Description | Objective: Considerable evidence links dietary salt intake with the development of hypertension, left ventricular hypertrophy, and increased risk of stroke and coronary heart disease. Despite extensive epidemiological and basic science interrogation of the relationship between high salt (HS) intake and blood pressure, it remains unclear how HS impacts endothelial cell (EC) and vascular structure in vivo. This study aims to uncover the HS-induced vascular pathologies using a differential systemic decellularization in vivo (DISDIVO) approach.
Approach and Results: We performed systematic molecular characterization of the endothelial glycocalyx (eGC) and EC proteomes in mice with HS (8%) diet-induced hypertension versus healthy control animals. Isolation of eGC and ECs compartments was achieved using the DISDIVO approach. Altered protein expression in hypertensive compared to normal mice was characterized by liquid chromatography tandem-mass spectrometry (LC-MS/MS). Proteomic results from eGC fractions revealed a significant downregulation of eGC and associated proteins in HS diet-induced hypertensive mice. Among 1696 proteins identified in this group, 723 were markedly downregulated while 168 were upregulated, bioinformatic analysis indicates critical damage and derangement of eGC layer. In the ECs fraction HS-induced hypertension significantly altered protein mediators of contractility, metabolism, mechanotransduction, renal functions, and coagulation cascades. In particular, we observed dysregulation of integrin subunits alpha2, alpha2b, and alpha5, which relay external signals to the actin cytoskeleton.
Conclusion: These findings provide novel molecular insight on HS-induced structure changes in eGC and ECs that may increase cardiovascular risk and potentially guide the development of new diagnostics and therapeutic interventions.
|
| HostingRepository | PRIDE |
| AnnounceDate | 2023-10-24 |
| AnnouncementXML | Submission_2023-10-24_13:34:20.461.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | SoFong CamNgan |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2022-01-25 00:55:28 | ID requested | |
| ⏵ 1 | 2023-10-24 13:34:21 | announced | |
| 2 | 2023-11-14 09:11:40 | announced | 2023-11-14: Updated project metadata. |
| 3 | 2024-10-22 06:12:45 | announced | 2024-10-22: Updated project metadata. |
Publication List
| Gallart-Palau X, Lorca C, Mulet M, S, á, nchez Mil, á, n JA, Lisa J, Ngan SC, Iyappan R, Katoueezadeh M, Serra A, Sze SK, vivo to study molecular changes in each vasculature layer in murine models of disease. STAR Protoc, 4(3):102524(2023) [pubmed] |
Keyword List
| submitter keyword: cardiovascular disease |
| endothelial cell dysfunction |
| glycocalyx |
| high salt diet |
| hypertension |
| mass spectrometry |
| proteomics |
Contact List
| Sze Sui Kwan,Newman |
|---|
| contact affiliation | Siu Kwan SZE, PhD, Associate professor School of Biological Sciences Division of Structural Biology and Biochemistry Nanyang Technological University, 60 Nanyang Drive, Singapore 637551 **Current affilliation Newman S-K. Sze, PhD Professor, Department of Health Sciences CIHR Tier1 Canada Research Chair in Mechanisms of Health and Disease Brock University | Faculty of Applied Health Sciences Niagara Region | 1812 Sir Isaac Brock Way | St. Catharines, ON L2S 3A1 |
| contact email | nsze@brocku.ca |
| lab head | |
| SoFong CamNgan |
|---|
| contact affiliation | Nanyang Technological University |
| contact email | sfcngan@ntu.edu.sg |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD031228
- Label: PRIDE project
- Name: Vascular bed systematic profiling unravels endothelial and glycocalyx dysregulations in experimental high salt hypertension
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