PXD031167 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparative proteome analysis of the tegument of male and female adult Schistosoma mansoni |
Description | We present a comparative analysis of sex-specific tegument proteins of paired or virgin Schistosoma mansoni. By apply a new and highly sensitive workflow, detection of even low abundance proteins from the worm was achieved Therefore, a streptavidin-biotin affinity purification technique in combination with single pot solid-phase enhanced sample preparation was established for subsequent LC-MS/MS analysis. We were able to identify 1519 tegument proteins for male and female as to virgin and paired worms. Sex-specific proteins were screened, present in both virgin and paired adult schistosomes to reduce the impact of mating. Bioinformatic analysis revealed an involvement of female-specific tegument proteins in signaling pathways of cellular processes and antioxidant mechanisms. Male-specific proteins were found to be enriched in processes linked to phosphorylation and signal transduction. This suggests a task sharing between the sexes that might be necessary for survival in the host. Our datasets provide a basis for further studies to understand and ultimately decipher the strategies of the two worm sexes to evade the immune system. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-20 |
AnnouncementXML | Submission_2022-05-20_05:52:12.056.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christian Hentschker |
SpeciesList | scientific name: Schistosoma mansoni; NCBI TaxID: 6183; |
ModificationList | biotinylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-01-21 13:21:47 | ID requested | |
⏵ 1 | 2022-05-20 05:52:12 | announced | |
Publication List
Winkelmann F, Gesell Salazar M, Hentschker C, Michalik S, Mach, á, č, ek T, Scharf C, Reisinger EC, V, ö, lker U, Sombetzki M, Comparative proteome analysis of the tegument of male and female adult Schistosoma mansoni. Sci Rep, 12(1):7569(2022) [pubmed] |
Keyword List
submitter keyword: Schistosoma manson, proteome, tegument |
Contact List
Martina Sombetzki |
contact affiliation | Division of Tropical Medicine and Infectious Diseases, Center of Internal Medicine II, Rostock University Medical Center, Rostock, Germany |
contact email | martina.sombetzki@uni-rostock.de |
lab head | |
Christian Hentschker |
contact affiliation | University Medicine Greifswald, Interfaculty Institute of Genetics and Functional Genomics, Department of Functional Genomics |
contact email | christian.hentschker@uni-greifswald.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD031167
- Label: PRIDE project
- Name: Comparative proteome analysis of the tegument of male and female adult Schistosoma mansoni