PXD031166-1

PXD031166 is an original dataset announced via ProteomeXchange.

Title	Evidence for a ‘trap-and-flip’ mechanism in a proton-dependent lipid transporter
Description	Lipid transporters from the major facilitator superfamily (MFS) have been reported to play central roles in diverse fundamental biological pathways. However, little is known about their transport mechanisms and only the structure of the MFS lipid transporter LtaA in an outward-facing conformation has been determined. LtaA was shown to be a proton-dependent lipid transporter, essential for anchoring of lipoteichoic acids (LTA) to the plasma membrane of the pathogenic bacteria Staphylococcus aureus. Here we used a ‘repeat swap’ approach in combination with cross-linking analysis to predict an inward-facing state of LtaA, performed molecular dynamics simulations, in vitro and in vivo activity assays, and mutagenesis analysis to characterize outward- and inward-facing conformations of LtaA in lipid membranes. Our results indicate that LtaA catalyze lipid translocation by a “trap-and-flip” mechanism, paving the way for the mechanistic characterization of other MFS lipid transporters.
HostingRepository	PRIDE
AnnounceDate	2022-05-16
AnnouncementXML	Submission_2022-05-16_15:04:05.710.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031166
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexander Schmidt
SpeciesList	scientific name: Staphylococcus aureus subsp. aureus NCTC 8325; NCBI TaxID: 93061;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-21 13:17:19	ID requested
⏵ 1	2022-05-16 15:04:06	announced

Dataset with its publication pending

submitter keyword: LtaA, targeted mass spectrometry

Alexander Schmidt
contact affiliation	Proteomics Core Facility, Biozentrum, University of Basel, Switzerland
contact email	alex.schmidt@unibas.ch
lab head
Alexander Schmidt
contact affiliation	Proteomics Core Facility
contact email	alex.schmidt@unibas.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031166
     1. Label: PRIDE project
     2. Name: Evidence for a ‘trap-and-flip’ mechanism in a proton-dependent lipid transporter