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PXD031166 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEvidence for a ‘trap-and-flip’ mechanism in a proton-dependent lipid transporter
DescriptionLipid transporters from the major facilitator superfamily (MFS) have been reported to play central roles in diverse fundamental biological pathways. However, little is known about their transport mechanisms and only the structure of the MFS lipid transporter LtaA in an outward-facing conformation has been determined. LtaA was shown to be a proton-dependent lipid transporter, essential for anchoring of lipoteichoic acids (LTA) to the plasma membrane of the pathogenic bacteria Staphylococcus aureus. Here we used a ‘repeat swap’ approach in combination with cross-linking analysis to predict an inward-facing state of LtaA, performed molecular dynamics simulations, in vitro and in vivo activity assays, and mutagenesis analysis to characterize outward- and inward-facing conformations of LtaA in lipid membranes. Our results indicate that LtaA catalyze lipid translocation by a “trap-and-flip” mechanism, paving the way for the mechanistic characterization of other MFS lipid transporters.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAlexander Schmidt
SpeciesList scientific name: Staphylococcus aureus subsp. aureus NCTC 8325; NCBI TaxID: 93061;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-21 13:17:19ID requested
12022-05-16 15:04:06announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: LtaA, targeted mass spectrometry
Contact List
Alexander Schmidt
contact affiliationProteomics Core Facility, Biozentrum, University of Basel, Switzerland
contact emailalex.schmidt@unibas.ch
lab head
Alexander Schmidt
contact affiliationProteomics Core Facility
contact emailalex.schmidt@unibas.ch
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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