PXD031115-1

PXD031115 is an original dataset announced via ProteomeXchange.

Title	Prenylcysteine oxidase 1 like protein is required for neutrophil bactericidal activities
Description	The bactericidal function of neutrophils are dependent on myriad intrinsic and extrinsic stimuli. Using systems immunology approaches we identified microbiome- and infection-induced changes in neutrophils. We focused on investigating the Prenylcysteine oxidase 1 like (Pcyox1l) protein function. Murine and human Pcyox1l proteins share ninety four percent aminoacid homology revealing significant evolutionary conservation and implicating Pcyox1l in mediating important biological functions. Here we show that the loss of Pcyox1l protein results in significant reductions in the mevalonate pathway impacting autophagy and cellular viability under homeostatic conditions. Concurrently, Pcyox1l CRISPRed-out neutrophils exhibit deficient bactericidal properties. Pcyox1l knock-out mice demonstrate significant susceptibility to infection with the gram-negative pathogen P. aeruginosa exemplified through increased neutrophil infiltrates, hemorrhaging, and reduced bactericidal functionality. Cumulatively, we ascribe a function to Pcyox1l protein as a fundamental regulator of the prenylation pathway and suggest connections beween metabolic responses and neutrophil functionality. Anastasia Petenkova , Shelby A. Auger, Jeffrey Lamb, Daisy Quellier, Cody Carter, On Tak To, Jelena Milosevic, Rana Barghout, Abirami Kugadas, Xiaoxiao Lu, Jennifer Geddes- McAlister, Raina Fichorova, David B. Sykes, Mark D. Distefano, and Mihaela Gadjeva. 2023. Prenylcysteine oxidase 1 like protein is required for neutrophil bactericidal activities. Nat.Comm.
HostingRepository	PRIDE
AnnounceDate	2023-04-14
AnnouncementXML	Submission_2023-04-13_16:17:58.049.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ShelbyAuger
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-20 09:17:46	ID requested
⏵ 1	2023-04-13 16:17:58	announced

Dataset with its publication pending

submitter keyword: Neutrophil, Prenylation, Pcyox1L

Mark, D,Distefano
contact affiliation	Chemistry Department, University of Minnesota
contact email	diste001@umn.edu
lab head
ShelbyAuger
contact affiliation	University of Minnesota
contact email	auger054@umn.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031115
     1. Label: PRIDE project
     2. Name: Prenylcysteine oxidase 1 like protein is required for neutrophil bactericidal activities