PXD031072-1

PXD031072 is an original dataset announced via ProteomeXchange.

Title	A plant tethering system for the functional study of protein-RNA interactions in vivo
Description	The sorting of RNA transcripts dictates their ultimate post-transcriptional fates, such as translation, decay or degradation by RNA interference (RNAi). This sorting of RNAs into distinct fates is mediated by their interaction with RNA-binding proteins. While hundreds of RNA binding proteins have been identified, which act to sort RNAs into different pathways is largely unknown. Particularly in plants, this is due to the lack of reliable protein-RNA artificial tethering tools necessary to determine the mechanism of protein action on an RNA in vivo. Here we used minimal Arabidopsis BRN1 RNA binding domains to generate a protein-RNA tethering system which functions on an endogenous Arabidopsis RNA called SOC1 that is tracked by the quantitative flowering time phenotype. Unlike other protein-RNA tethering systems that have been attempted in plants, our system circumvents the inadvertent triggering of RNAi. We successfully in vivo tethered a protein epitope to the RNA and identified new proteins that were previously unknown to interact during BRN1-RNA binding. This protein-RNA tethering system enables the future determination of any protein’s function upon recruitment to an RNA and can be used to discover new interactions with RNA-binding proteins.
HostingRepository	PRIDE
AnnounceDate	2022-05-10
AnnouncementXML	Submission_2022-05-09_16:23:30.755.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031072
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yu-Hung Hung
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-19 03:01:02	ID requested
⏵ 1	2022-05-09 16:23:31	announced

Dataset with its publication pending

submitter keyword: Arabidopsis, RNA binding, IP-MS, Bruno1

R. Keith Slotkin
contact affiliation	Donald Danforth Plant Science Center, St. Louis, MO, USA Division of Biological Sciences, University of Missouri, Columbia, MO, USA
contact email	KSlotkin@danforthcenter.org
lab head
Yu-Hung Hung
contact affiliation	Donald Danforth Plant Science Center
contact email	yhung@danforthcenter.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD031072

PRIDE project URI

• PRIDE
  1. PXD031072
     1. Label: PRIDE project
     2. Name: A plant tethering system for the functional study of protein-RNA interactions in vivo