PXD031067 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The fate of bioaccessible coeliac toxic motifs and IgE epitopes during simulated gastro-intestinal digestion of pizza base |
Description | Understanding how food processing may modify allergen bioaccessibility and the evolution of immunologically active peptides in the gastrointestinal tract is essential if knowledge-based ap-proaches to reducing the allergenicity of food is to be realised. A soy-enriched wheat-based pizza base was subjected to in vitro oral-gastro-duodenal digestion and resulting digests analysed using a combination of SDS-PAGE and mass spectrometry (MS). The digestion profile of pizza base resembled that of bread crust where higher temperatures during baking reduced protein solu-bility but still resulted in the generation of a complex mixture of peptides. MS profiling showed of the peptides carried IgE-epitopes and coeliac toxic motifs many of which were in excess of 20-30 residues long which were only released after extended (120 min) gastric digestion or a combina-tion of gastric and duodenal digestion. Comparison with in silico prediction tools showed that they either under-estimated pepsinolysis products or over estimated tryptic/chymotryptic cleavage patterns. These data show that simple thermal treatments, such as baking, are insuffi-cient to destroy the allergenic potential of wheat and soybean proteins. The findings contribute to efforts to provide bench marks for mapping in vitro digestion products of novel proteins as part of the allergenicity risk assessment. |
HostingRepository | PRIDE |
AnnounceDate | 2022-08-12 |
AnnouncementXML | Submission_2022-08-11_23:37:45.082.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Matthew Daly |
SpeciesList | scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565; scientific name: Glycine max; NCBI TaxID: 3847; |
ModificationList | monohydroxylated residue; monohydroxylated proline; iodoacetamide derivatized residue; deamidated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-01-19 02:40:28 | ID requested | |
⏵ 1 | 2022-08-11 23:37:45 | announced | |
Publication List
Daly ME, Wang K, Pan X, Depau RL, Marsh J, Capozzi F, Johnson P, Gethings LA, Mills ENC, The Fate of IgE Epitopes and Coeliac Toxic Motifs during Simulated Gastrointestinal Digestion of Pizza Base. Foods, 11(14):(2022) [pubmed] |
Keyword List
submitter keyword: Wheat, gluten, soy, allergen, coeliac toxic motif, IgE epitope, in vitro digestion, mass spectrome-try, allergenicity risk assessment. |
Contact List
Clare Mills |
contact affiliation | Manchester Institute of Biotechnology, School of Biological Sciences, Manchester Academic Health Sciences Centre, The University of Manchester, Princess Street, Manchester, M1 7DN, UK |
contact email | clare.mills@manchester.ac.uk |
lab head | |
Matthew Daly |
contact affiliation | University of Manchester |
contact email | matthew.daly-3@postgrad.manchester.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD031067
- Label: PRIDE project
- Name: The fate of bioaccessible coeliac toxic motifs and IgE epitopes during simulated gastro-intestinal digestion of pizza base