PXD031063-1

PXD031063 is an original dataset announced via ProteomeXchange.

Title	ABP1-TMK auxin perception mediates rapid phosphorylation for regeneration and auxin canalization
Description	The phytohormone auxin triggers transcriptional reprograming utilizing a well-characterized nuclear perception machinery. In contrast, mechanisms underlying other auxin effects, such as auxin feed-back on its transport, rapid regulation of ion fluxes or ultrafast global phospho-response, remain enigmatic. Auxin Binding Protein 1 (ABP1) has been contested as an auxin receptor candidate since decades. Here we show that a fraction of Arabidopsis thaliana ABP1 is secreted and binds auxin specifically at the acidic pH typical for the apoplast. ABP1 and its plasma membrane-localized partner, Transmembrane Kinase 1 (TMK1) are required for auxin-induced phosphorylation of about thousand proteins. Loss-of-function abp1 alleles but not complemented lines show defects in in vitro shoot regeneration and formation of auxin-transporting channels for vasculature formation and regeneration. These results support a role of ABP1-TMK1 in cell surface auxin perception mediating global auxin phospho-response and regenerative development.
HostingRepository	PRIDE
AnnounceDate	2022-08-25
AnnouncementXML	Submission_2022-08-25_03:50:37.739.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mark Roosjen
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-19 02:36:06	ID requested
⏵ 1	2022-08-25 03:50:38	announced

Dataset with its publication pending

submitter keyword: Auxin ABP1 TMK1 phosphoproteomics

Prof.Dr. Dolf Weijers
contact affiliation	Department head
contact email	dolf.weijers@wur.nl
lab head
Mark Roosjen
contact affiliation	Wageningen University and Research
contact email	mark.roosjen@wur.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD031063

PRIDE project URI

• PRIDE
  1. PXD031063
     1. Label: PRIDE project
     2. Name: ABP1-TMK auxin perception mediates rapid phosphorylation for regeneration and auxin canalization