PXD031041-1

PXD031041 is an original dataset announced via ProteomeXchange.

Title	Effects of plasticizers on human preadipocytes (SGBS) using global proteomics
Description	SGBS cells were used as a model system to investigate the cellular mode of action of a variety of plasticizers in human adipocytes. In brief, cells were cultured at 5% CO2 and 37°C in 95% humidity. Cells of generation 40 and passage 3 after thawing were grown to confluence with DMEM/F12 containing 33 µM biotin, 17 µM pantothenate, 100 U/l penicillin, and 0.1 mg/l streptomycin (basal medium) supplemented with 10% FCS (Gibco, Carlsbad, CA, USA). According to the standard differentiation protocol, differentiation was initiated (day 0) after changing to serum-free basal medium supplemented with 0.1 μM cortisol, 0.01 mg/ml apo-transferrin, 0.2 nM triiodothyronine, and 20 nM human insulin (differentiation medium) with the addition of 2 µM rosiglitazone, 25 nM dexamethasone, and 200 µM 3-isobutyl-1-methylxanthine for the first four days. The positive control was differentiated using the standard protocol with rosiglitazone. For plasticizer treatments, differentiation was conducted without rosiglitazone and cells were continuously exposed from day 0 – day 16 to the plasticizers or their transformation products. As for plasticizer treatments, the negative control was differentiated with rosiglitazone-free medium containing equivalent amounts of solvent (0.01 MeOH, v/v) for 16 days, resulting in minimal differentiation. All media were renewed every second day to mimic continuous exposure.
HostingRepository	PRIDE
AnnounceDate	2022-06-20
AnnouncementXML	Submission_2022-06-19_21:18:34.519.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031041
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexandra Schaffert
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-18 02:01:58	ID requested
⏵ 1	2022-06-19 21:18:34	announced
2	2023-11-14 08:54:13	announced	2023-11-14: Updated project metadata.

10.1016/J.ENVINT.2022.107279;

submitter keyword: TMT, SGBS, adipocytes, xenobiotics, PPARgamma, endocrine disruption

Kristin Schubert
contact affiliation	Department of Molecular Systems Biology, Helmholtz Centre for Environmental Research (UFZ), Leipzig, Germany
contact email	kristin.schubert@ufz.de
lab head
Alexandra Schaffert
contact affiliation	Helmholtz-Centre for Environmental Research
contact email	alexandra.schaffert@ufz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD031041

PRIDE project URI

• PRIDE
  1. PXD031041
     1. Label: PRIDE project
     2. Name: Effects of plasticizers on human preadipocytes (SGBS) using global proteomics