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PXD031041-1

PXD031041 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEffects of plasticizers on human preadipocytes (SGBS) using global proteomics
DescriptionSGBS cells were used as a model system to investigate the cellular mode of action of a variety of plasticizers in human adipocytes. In brief, cells were cultured at 5% CO2 and 37°C in 95% humidity. Cells of generation 40 and passage 3 after thawing were grown to confluence with DMEM/F12 containing 33 µM biotin, 17 µM pantothenate, 100 U/l penicillin, and 0.1 mg/l streptomycin (basal medium) supplemented with 10% FCS (Gibco, Carlsbad, CA, USA). According to the standard differentiation protocol, differentiation was initiated (day 0) after changing to serum-free basal medium supplemented with 0.1 μM cortisol, 0.01 mg/ml apo-transferrin, 0.2 nM triiodothyronine, and 20 nM human insulin (differentiation medium) with the addition of 2 µM rosiglitazone, 25 nM dexamethasone, and 200 µM 3-isobutyl-1-methylxanthine for the first four days. The positive control was differentiated using the standard protocol with rosiglitazone. For plasticizer treatments, differentiation was conducted without rosiglitazone and cells were continuously exposed from day 0 – day 16 to the plasticizers or their transformation products. As for plasticizer treatments, the negative control was differentiated with rosiglitazone-free medium containing equivalent amounts of solvent (0.01 MeOH, v/v) for 16 days, resulting in minimal differentiation. All media were renewed every second day to mimic continuous exposure.
HostingRepositoryPRIDE
AnnounceDate2022-06-20
AnnouncementXMLSubmission_2022-06-19_21:18:34.519.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD031041
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAlexandra Schaffert
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-18 02:01:58ID requested
12022-06-19 21:18:34announced
22023-11-14 08:54:13announced2023-11-14: Updated project metadata.
Publication List
10.1016/J.ENVINT.2022.107279;
Keyword List
submitter keyword: TMT, SGBS, adipocytes, xenobiotics, PPARgamma, endocrine disruption
Contact List
Kristin Schubert
contact affiliationDepartment of Molecular Systems Biology, Helmholtz Centre for Environmental Research (UFZ), Leipzig, Germany
contact emailkristin.schubert@ufz.de
lab head
Alexandra Schaffert
contact affiliationHelmholtz-Centre for Environmental Research
contact emailalexandra.schaffert@ufz.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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