PXD030995-1

PXD030995 is an original dataset announced via ProteomeXchange.

Title	Metabolic control of adult neural stem cell self-renewal by the mitochondrial protease YME1L
Description	The transition between quiescence and activation in neural stem and progenitor cells (NSPCs) is coupled to reversible changes in energy metabolism with key implications for life-long NSPC self-renewal and neurogenesis. How this metabolic plasticity is ensured between NSPC activity states is unclear. We find that a state-specific rewiring of the mitochondrial proteome by the i-AAA peptidase YME1L is required to preserve NSPC self-renewal. YME1L controls the abundance of numerous mitochondrial substrates in quiescent NSPCs, and its deletion activates a differentiation program characterized by broad metabolic changes causing the irreversible shift away from a fatty acid oxidation-dependent state. Conditional Yme1l deletion in adult NSPCs in vivo results in defective self-renewal and premature differentiation, ultimately leading to NSPC pool depletion. Our results disclose an important role for YME1L in coordinating the switch between metabolic states of NSPCs and suggest that NSPC fate is regulated by compartmentalized changes in protein network dynamics.
HostingRepository	PRIDE
AnnounceDate	2022-02-10
AnnouncementXML	Submission_2022-02-10_00:14:15.543.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Prerana Wagle
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-16 13:11:24	ID requested
⏵ 1	2022-02-10 00:14:15	announced

Dataset with its publication pending

submitter keyword: neural stem cells

Mitochondria

metabolic rewiring

mitochondrial proteome

mitochondrial dynamics

adult neurogenesis

Self-renewal

YME1L

OMA1

Matteo Bergami
contact affiliation	Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases
contact email	matteo.bergami@uk-koeln.de
lab head
Prerana Wagle
contact affiliation	CECAD Research Center
contact email	proteomics-facility@uni-koeln.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030995
     1. Label: PRIDE project
     2. Name: Metabolic control of adult neural stem cell self-renewal by the mitochondrial protease YME1L