PXD030991 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | CK2β is a gatekeeper of focal adhesions regulating cell spreading |
| Description | CK2 is an hetero-tetrameric protein made of two CK2α catalytic subunits and two regulatory CK2β subunits. The free CK2α subunit and the tetrameric holoenzyme have distinct substrate specificity profiles suggesting that the spatiotemporal organization of the individual CK2 subunits observed in living cells is crucial in the control of the many cellular processes that are governed by this pleiotropic kinase. Indeed, sub-stoichiometric expression of CK2β compared to CK2α in a subset of breast cancer tumors was correlated with the induction of EMT markers and increased epithelial cell plasticity in breast carcinoma progression. Using phosphotyrosine profiling with affinity capture and LC/MS-MS, we show that decreased expression of CK2β in MCF10A mammary epithelial cells triggers the phosphorylation of a number of tyrosine phosphorylated proteins and promotes the striking activation of the FAK1-Src-PAX1 signaling pathway. Moreover, morphometric analyses also revealed that CK2β loss increases the number and the spatial distribution of focal adhesion signaling complexes. Together, our finding allows positioning CK2β as a gatekeeper for cell spreading by restraining the focal adhesion maturation and invasion of mammary epithelial cells. |
| HostingRepository | PRIDE |
| AnnounceDate | 2022-07-05 |
| AnnouncementXML | Submission_2022-07-05_02:14:59.320.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD030991 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Hesse Anne-Marie |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2022-01-16 09:30:35 | ID requested | |
| ⏵ 1 | 2022-07-05 02:14:59 | announced | |
| 2 | 2023-11-14 08:55:07 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
| submitter keyword: CK2β depletion, Tyrosine phosphorylated proteins, LC-MS/MS, proteomics |
Contact List
| Yohann Couté |
|---|
| contact affiliation | Univ. Grenoble Alpes, INSERM, CEA, UMR BioSanté U1292, CNRS, CEA, FR2048, 38000 Grenoble, France |
| contact email | yohann.coute@cea.fr |
| lab head | |
| Hesse Anne-Marie |
|---|
| contact affiliation | Laboratoire d'Etude de la Dynamique des Protéomes |
| contact email | anne-marie.hesse@cea.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD030991 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030991
- Label: PRIDE project
- Name: CK2β is a gatekeeper of focal adhesions regulating cell spreading
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