PXD030926-3

PXD030926 is an original dataset announced via ProteomeXchange.

Title	LC-MS/MS clams and cockle mantle data
Description	Increased heavy rainfall cause important drops in salinity to values close to 0, which can persist for several days in estuaries. Lethal and sublethal physiological and behavioural effects of decreases in salinity below ten have already been found to occur in the commercially relevant clam species Venerupis corrugata, Ruditapes decussatus and R. philippinarum and the cockle Cerastoderma edule, which generate ~74 million euros annually in Galicia (NW Spain). However, studies of the molecular response to hyposaline stress in bivalves are scarce. This ‘shotgun’ proteomics study evaluates changes in mantle-edge proteins subjected to short-term hyposaline episodes in two different months (March and May) during the gametogenic cycle. We found evidence that the effects of the gametogenic cycle on the proteome are greater than those related to the salinity treatments. However, hyposalinity modulated proteome profiles for both months in V. corrugata and C. edule and on R. philippinarum in May, involving proteins implicated in ROS production, redox homeostasis, cytoskeleton modulation and the activation of apoptotic, autophagic and lipid degradation pathways. Nevertheless, essential proteins for an optimal osmotic stress response but high energy demandants, such as chaperones, osmoprotectants and DNA repair factors, were in both large and small abundance under hyposalinity for those species. In both time points for R. decussatus and R. philippinarum in March, almost no differences between treatments were detected. These exploratory results reinforce the interest in the specific study and conservation of native species C. edule and V. corrugata, particularly sensitive to hyposalinity.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:39:52.435.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sofía Blanco
SpeciesList	scientific name: Ruditapes philippinarum; NCBI TaxID: NCBITaxon:129788; scientific name: Cerastoderma edule; NCBI TaxID: NCBITaxon:55710; scientific name: Venerupis corrugata; NCBI TaxID: 1028563; scientific name: Ruditapes decussatus; NCBI TaxID: NCBITaxon:104385;
ModificationList	methionine oxidation with neutral loss of 64 Da; mono N-acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-13 01:39:52	ID requested
1	2022-09-28 11:24:58	announced
2	2023-11-14 08:57:15	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:39:52	announced	2024-10-22: Updated project metadata.

10.1016/J.ENVRES.2022.114371;

submitter keyword: salinity, stress,clam, cockle, proteome

Ángel E. Pérez Diz
contact affiliation	Universidade de Vigo
contact email	angel.p.diz@uvigo.es
lab head
Sofía Blanco
contact affiliation	University of Vigo
contact email	sofiablancoglez@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD030926

PRIDE project URI

• PRIDE
  1. PXD030926
     1. Label: PRIDE project
     2. Name: LC-MS/MS clams and cockle mantle data