PXD030915-1

PXD030915 is an original dataset announced via ProteomeXchange.

Title	Spatiotemporal profiling of the bovine oviduct fluid proteome around the time of ovulation
Description	Unraveling the composition and regulation of the oviduct fluid (OF) is crucial to understand the microenvironment in which sperm capacitation, fertilization and early embryo development take place. Therefore, our aim was to determine the spatiotemporal changes in the OF proteome according to the anatomical region of the oviduct (ampulla vs. isthmus), the proximity of the ovulating ovary (ipsilateral vs. contralateral side) and the peri-ovulatory stage (pre-ovulatory or Pre-ov vs. post-ovulatory or Post-ov). Oviducts from adult cyclic cows were collected at a local slaughterhouse and pools of OF were analyzed by nanoLC-MS/MS and label-free protein quantification (n=32 OF pools for all region  stage  side conditions). A total of 3,760 proteins were identified in the OF, of which 37% were predicted to be secreted. The oviduct region was the major source of variation in protein abundance, followed by the proximity of the ovulating ovary and finally the peri-ovulatory stage. Differentially abundant proteins between regions, stages and sides were involved in a broad variety of biological functions, including protein binding, response to stress, cell-to-cell adhesion, calcium homeostasis and the immune system. This work highlights the dynamic regulation of oviduct secretions and provides new protein candidates for interactions between the maternal environment, the gametes and the early embryo.
HostingRepository	PRIDE
AnnounceDate	2022-03-03
AnnouncementXML	Submission_2022-03-03_03:38:12.904.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD030915
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	E Com
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-12 08:59:19	ID requested
⏵ 1	2022-03-03 03:38:13	announced

Dataset with its publication pending

submitter keyword: oviduct fluid, proteomics, mass spectrometry, ampulla, isthmus, pre-ovulatory, post-ovulatory, ipsilateral, contralateral, cattle

Charles Pineau
contact affiliation	UMS Biosit - CNRS 3480 - Inserm 018 Campus de Beaulieu - Bâtiment. 24 - CS2407 263 Avenue du Général Leclerc 35042 RENNES CEDEX FRANCE
contact email	proteome@univ-rennes1.fr
lab head
E Com
contact affiliation	Protim, Irset, Inserm U1085
contact email	emmanuelle.com@univ-rennes1.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030915
     1. Label: PRIDE project
     2. Name: Spatiotemporal profiling of the bovine oviduct fluid proteome around the time of ovulation