PXD030901 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteins are well-preserved in shells toasted at 300°C revealed by proteomics |
Description | The development of protein anti-degradation strategy is important for their storage at ambient conditions. Despite that it is known that biominerals, typical inorganic-organic composites, can preserve proteins at room temperature for a long time, it is unclear the extent of protein degradation under high temperatures. In this study, we examined protein remaining in the toasted abalone shell under high temperatures (200 and 300 °C) by biomineral proteomics method. Surprisingly, 24 proteins including carbonic anhydrase, hemocyanin, actin can still be identified from shells even after toasting under 300°C, not much decreased compared to that in the 200°C-treated and the native shell. However, the microstructure and composition (both mineral and organic matrix) of shells were altered significantly revealed by scanning electron microscopy, infrared spectroscopy, and X-ray diffraction. The well-preserved of proteins may be partially due to the sacrifice of mineral/organic interfaces and the formation of nanopores in the shell at high temperatures. Moreover, the extracted proteins from both groups were able to affect calcium carbonate in vitro. This study has potential implications in various fields such as protein storage at high temperatures and palaeoproteomics. |
HostingRepository | PRIDE |
AnnounceDate | 2022-07-01 |
AnnouncementXML | Submission_2022-06-30_20:19:39.357.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Chuang Liu |
SpeciesList | scientific name: Haliotis discus hannai; NCBI TaxID: 42344; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-01-12 00:21:51 | ID requested | |
⏵ 1 | 2022-06-30 20:19:39 | announced | |
2 | 2023-11-14 08:31:35 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: shell matrix proteins |
biomineralization |
high temperature |
Contact List
Chuang Liu |
contact affiliation | Hohai University, Nanjing, China |
contact email | helloliuchuang@163.com |
lab head | |
Chuang Liu |
contact affiliation | Hohai University, Nanjing, China |
contact email | helloliuchuang@163.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030901
- Label: PRIDE project
- Name: Proteins are well-preserved in shells toasted at 300°C revealed by proteomics