PXD030886-1

PXD030886 is an original dataset announced via ProteomeXchange.

Title	Proteomic and functional analyses of the periodic membrane skeleton in neurons
Description	Actin, spectrin, and associated proteins form a membrane-associated periodic skeleton (MPS) in neurons. The molecular composition and functions of the MPS remain incompletely understood. Here, we combined co-immunoprecipitation and mass spectrometry analyses to identify candidate MPS-interacting proteins from cultured hippocampal neurons and adult mouse brains. In addition, we also used quantitative mass spectrometry analysis based on Tandem Mass Tag (TMT) isobaric labeling to determine how the expression levels of proteins are differentially regulated at the proteomic scale upon depletion of βII-spectrin, an important molecular component of the MPS. These results provide a systematic picture of the interactome of the MPS, and new insights into new functions of the MPS in neurons.
HostingRepository	PRIDE
AnnounceDate	2022-05-04
AnnouncementXML	Submission_2022-05-04_04:41:45.297.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ruobo Zhou
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-11 01:26:24	ID requested
⏵ 1	2022-05-04 04:41:45	announced

Dataset with its publication pending

submitter keyword: Actin, spectrin, membrane skeleton, neuron, proteomics

Xiaowei Zhuang
contact affiliation	Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Department of Physics, Harvard University, Cambridge, MA 02138, USA
contact email	zhuang@chemistry.harvard.edu
lab head
Ruobo Zhou
contact affiliation	The Pennsylvania State University
contact email	ruobo.zhou@psu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD030886

PRIDE project URI

• PRIDE
  1. PXD030886
     1. Label: PRIDE project
     2. Name: Proteomic and functional analyses of the periodic membrane skeleton in neurons