PXD030870-1
PXD030870 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic screen reveals diverse protein transport between connected neurons in the visual system. |
| Description | Intercellular transfer of toxic proteins between neurons is thought to contribute to neurodegenerative disease, however whether direct inter-neuronal protein transfer occurs in the healthy brain is not clear. To assess the prevalence and identity of transferred proteins and the cellular specificity of transfer, we biotinylated retinal ganglion cell proteins in vivo and examined biotinylated proteins transported through the rodent visual circuit using microscopy, biochemistry, and mass spectrometry. Electron microscopy demonstrated preferential transfer of biotinylated proteins from retinogeniculate inputs to excitatory LGN neurons compared to GABAergic neurons. An unbiased mass spectrometry-based screen identified 200 transneuronally transported proteins (TNTPs) isolated from visual cortex. The majority of TNTPs are present in neuronal exosomes and virally-expressed TNTPs, including tau and beta-synuclein, were detected in isolated exosomes and postsynaptic neurons. Our data demonstrate transfer of diverse endogenous proteins between neurons in the healthy intact brain and suggest that TNTP transport may be mediated by exosomes. |
| HostingRepository | MassIVE |
| AnnounceDate | 2022-01-10 |
| AnnouncementXML | Submission_2022-01-10_11:15:40.815.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Daniel McClatchy |
| SpeciesList | scientific name: Rattus norvegicus; common name: Norway rat; NCBI TaxID: 10116; |
| ModificationList | Biotin; TMT6plex |
| Instrument | LTQ Velos; LTQ Orbitrap Elite; Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-01-10 10:56:53 | ID requested | |
| ⏵ 1 | 2022-01-10 11:15:41 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: tranpsort, neurons |
Contact List
| Hollis T. Cline | |
|---|---|
| contact affiliation | The Scripps Research Institute |
| contact email | cline@scripps.edu |
| lab head | |
| Daniel McClatchy | |
| contact affiliation | TSRI |
| contact email | dmcclat@scripps.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000088669/ |
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