PXD030854-1

PXD030854 is an original dataset announced via ProteomeXchange.

Title	Proteomic insight into soybean response to flooding stress reveals changes in energy metabolism and cell wall modifications
Description	Soybean is a legume crop enriched with proteins and oil. It is frequently exposed to anthropogenic and natural flooding that limits its growth and yield. The current study applied gel-free proteomic techniques to unravel soybean response mechanisms to flooding stress. Two-day-old soybeans were flooded for 4 days continuously, and root samples were collected on days 2 to 6 for proteomic and enzymatic analyses. Age-matched untreated soybeans were collected as a control. After protein extraction, purification, and tryptic digestion, the peptides were analyzed on nano-liquid chromatography-mass spectrometry. A total of 539 and 472 proteins with matched peptides 2 or more were identified in control and flooded seedlings, respectively. Among these 364 proteins were commonly identified in both control and flooded soybeans. Fourty-two protein’s abundances were changed 4-fold after 2-days of flooding stress as compared to starting point. The cluster analysis showed that highly increased proteins included cupin family proteins, enolase, pectin methylesterase inhibitor, glyoxalase II, alcohol dehydrogenase, and aldolase. The enzyme assay of enolase and pectin methylesterase inhibitor confirmed protein abundance changes. These findings suggest that soybean adopts the less energy-consuming strategies and brings biochemical and structural changes in the cell wall to effectively respond to flooding stress and for survival.
HostingRepository	iProX
AnnounceDate	2022-01-09
AnnouncementXML	Submission_2022-03-27_19:29:38.194.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mudassar Nawaz Khan
SpeciesList	scientific name: Glycine max; NCBI TaxID: 3847;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-09 21:14:55	ID requested
⏵ 1	2022-03-27 19:29:38	announced

Dataset with its publication pending

submitter keyword: Soybean, flooding, proteomic, cell wall, enolase

Mudassar Nawaz Khan
contact affiliation	Hazara University Mansehra, Pakistan
contact email	mnkhan@hu.edu.pk
lab head
Mudassar Nawaz Khan
contact affiliation	Hazara University Mansehra, Pakistan
contact email	mnkhan@hu.edu.pk
dataset submitter

iProX dataset URI