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PXD030776-1

PXD030776 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe proto-oncogene TCL1A deregulates mitotic checkpoint transition and genomic stability in CLL
DescriptionUpregulation of the proto-oncogene TCL1A is causally implicated in various B- and T-cell malignancies. High-level TCL1A correlates with aggressive disease features and inferior clinical outcomes. However, molecular and cell-biological consequences of TCL1A dysregulation are not fully elucidated. Here, we identify CDC20 and other molecules of the safeguarding cell cycle checkpoints as novel TCL1A-interactors. In different model systems of B-cell leukemia/lymphoma, TCL1A overexpression accelerated cell cycle transition, impaired apoptotic damage responses in association with pronounced chromosome mis-segregation and caused cellular aneuploidy. In primary CLL samples, low CDC20 expression correlated with high expression of TCL1A and more aggressive disease characteristics. In line, a low CDC20 expression was a marker for reduced progression-free survival in CLL patients. Finally, knockdown of CDC20 in TCL1A-initiated murine lymphomas promoted chromosome disbalances and leukemia outgrowth. Overall, we discovered a cell-cycle associated effect of TCL1A by interfering with a proficient cell cycle transition. This adds to our concept of TCL1A’s transforming impact by targeting genome stability.
HostingRepositoryPRIDE
AnnounceDate2022-11-14
AnnouncementXMLSubmission_2022-11-14_03:35:57.906.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPreranaWagle
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-06 01:40:48ID requested
12022-11-14 03:35:58announced
22023-11-14 08:54:48announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: TCL1A, leukemia, CDC20, mitotic checkpoint, aneuploidy
Contact List
MarcoHerling
contact affiliation1. Department I of Internal Medicine, Center for Integrated Oncology (CIO), Aachen-Bonn- Cologne-Duesseldorf, Excellence Cluster for Cellular Stress Response and Aging-Associated Diseases (CECAD), Center for Molecular Medicine Cologne (CMMC), University of Cologne (UoC), 50937 Cologne, Germany 2. Department of Hematology and Cellular Therapy, University of Leipzig, Leipzig, Germany
contact emailmarco.herling@medizin.uni-leipzig.de
lab head
PreranaWagle
contact affiliationCECAD Research Center
contact emailproteomics-facility@uni-koeln.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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