PXD030776 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The proto-oncogene TCL1A deregulates mitotic checkpoint transition and genomic stability in CLL |
Description | Upregulation of the proto-oncogene TCL1A is causally implicated in various B- and T-cell malignancies. High-level TCL1A correlates with aggressive disease features and inferior clinical outcomes. However, molecular and cell-biological consequences of TCL1A dysregulation are not fully elucidated. Here, we identify CDC20 and other molecules of the safeguarding cell cycle checkpoints as novel TCL1A-interactors. In different model systems of B-cell leukemia/lymphoma, TCL1A overexpression accelerated cell cycle transition, impaired apoptotic damage responses in association with pronounced chromosome mis-segregation and caused cellular aneuploidy. In primary CLL samples, low CDC20 expression correlated with high expression of TCL1A and more aggressive disease characteristics. In line, a low CDC20 expression was a marker for reduced progression-free survival in CLL patients. Finally, knockdown of CDC20 in TCL1A-initiated murine lymphomas promoted chromosome disbalances and leukemia outgrowth. Overall, we discovered a cell-cycle associated effect of TCL1A by interfering with a proficient cell cycle transition. This adds to our concept of TCL1A’s transforming impact by targeting genome stability. |
HostingRepository | PRIDE |
AnnounceDate | 2022-11-14 |
AnnouncementXML | Submission_2022-11-14_03:35:57.906.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | PreranaWagle |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-01-06 01:40:48 | ID requested | |
⏵ 1 | 2022-11-14 03:35:58 | announced | |
2 | 2023-11-14 08:54:48 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: TCL1A, leukemia, CDC20, mitotic checkpoint, aneuploidy |
Contact List
MarcoHerling |
contact affiliation | 1. Department I of Internal Medicine, Center for Integrated Oncology (CIO), Aachen-Bonn- Cologne-Duesseldorf, Excellence Cluster for Cellular Stress Response and Aging-Associated Diseases (CECAD), Center for Molecular Medicine Cologne (CMMC), University of Cologne (UoC), 50937 Cologne, Germany 2. Department of Hematology and Cellular Therapy, University of Leipzig, Leipzig, Germany |
contact email | marco.herling@medizin.uni-leipzig.de |
lab head | |
PreranaWagle |
contact affiliation | CECAD Research Center |
contact email | proteomics-facility@uni-koeln.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030776
- Label: PRIDE project
- Name: The proto-oncogene TCL1A deregulates mitotic checkpoint transition and genomic stability in CLL