PXD030752-1

PXD030752 is an original dataset announced via ProteomeXchange.

Title	Signaling mechanisms of the prototypical psychedelic LSD
Description	Serotonin (5-hydroxytryptamine; 5-HT) 5-HT2-family receptors are the key targets of the prototypical psychedelic LSD. Although the canonical signaling pathway for 5-HT2 receptors is mediated by Gq protein, recent studies suggest that G-protein independent signaling, namely β-arrestin signaling pathway, may be also essential for actions of LSD in vitro and in vivo. To gain comprehensive molecular insights into the signaling mechanisms of LSD, we determined the cryoEM structures of LSD-bound 5-HT2BR in transducer-free state, G protein-coupled state and β-arrestin-1-coupled state, respectively. Besides, we use mass spectrometry to determine the phosphorylation sites of LSD-bound 5-HT2BR.
HostingRepository	PRIDE
AnnounceDate	2023-10-24
AnnouncementXML	Submission_2023-10-24_10:21:27.775.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	XiaofangZhong
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-05 13:05:12	ID requested
⏵ 1	2023-10-24 10:21:28	announced
2	2023-11-14 09:06:31	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:07:50	announced	2024-10-22: Updated project metadata.

Cao C, Barros-, Á, lvarez X, Zhang S, Kim K, D, ä, mgen MA, Panova O, Suomivuori CM, Fay JF, Zhong X, Krumm BE, Gumpper RH, Seven AB, Robertson MJ, Krogan NJ, H, ü, ttenhain R, Nichols DE, Dror RO, Skiniotis G, Roth BL, Signaling snapshots of a serotonin receptor activated by the prototypical psychedelic LSD. Neuron, 110(19):3154-3167.e7(2022) [pubmed]

submitter keyword: LC-MSMS

RuthHuttenhain
contact affiliation	University of California-San Francisco
contact email	Ruth.Huttenhain@ucsf.edu
lab head
XiaofangZhong
contact affiliation	University of Wisconsin-Madison
contact email	xzhong9@wisc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD030752

PRIDE project URI

• PRIDE
  1. PXD030752
     1. Label: PRIDE project
     2. Name: Signaling mechanisms of the prototypical psychedelic LSD