PXD030750-1

PXD030750 is an original dataset announced via ProteomeXchange.

Title	Comparison of wild type and abc1k6-1 mutant total leaf extracts from 4 and 5 week old plants
Description	The quantitative proteomes of leaf tissue from A. thaliana at 4 weeks and 5 weeks of growth using tandem mass spectrometry with label-free quantification. A comparison of three genotypes has been performed, namely i) the Col-0 wild type ecotype, ii) a T-DNA insertion mutant disrupting the plastoglobule-localized ABC1K6 protein (abc1k6-1) that is delayed in transition to reproductive growth, and iii) a complementation line (Comp3) that restores the timely progression of development with the ABC1K6 wild-type protein sequence tagged C-terminally with GFP in the abc1k6-1 background. Whereas wild-type and abc1k6-1 remain in the vegetative growth stage at 4 weeks of age, the wild-type plants have transitioned to reproductive growth (as evidenced by the emergence of the infloresence bolt) by 5 weeks of age, while abc1k6-1 remians in vegetative growth. Our proteomics experiment seeks to identify leaf proteome differences prior to wild-type transition to reproductive growth that may account for the delayed transition in the abc1k6-1 mutant.
HostingRepository	PRIDE
AnnounceDate	2022-03-02
AnnouncementXML	Submission_2022-03-02_12:05:26.692.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Lundquist
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-05 13:00:26	ID requested
⏵ 1	2022-03-02 12:05:27	announced
2	2023-11-14 08:54:13	announced	2023-11-14: Updated project metadata.

10.1016/J.JBC.2022.101762;

submitter keyword: leaf, abc1k6, coq8

Peter Lundquist
contact affiliation	Department of Biochemistry & Molecular Biology, Michigan State University
contact email	pklundqu@msu.edu
lab head
Peter Lundquist
contact affiliation	Michigan State University
contact email	PKLUNDQU@MSU.EDU
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD030750

PRIDE project URI

• PRIDE
  1. PXD030750
     1. Label: PRIDE project
     2. Name: Comparison of wild type and abc1k6-1 mutant total leaf extracts from 4 and 5 week old plants