PXD030734-1

PXD030734 is an original dataset announced via ProteomeXchange.

Title	DOT1L regulates chromatin reorganization and gene expression during sperm differentiation
Description	Spermatozoa have a unique genome organization: their chromatin is almost completely devoid of histones and is formed instead of protamines which confer a high level of compaction and preserve paternal genome integrity until fertilization. Histone-to-protamine transition takes place in spermatids and is indispensable for the production of functional sperm. Here we show that the H3K79-methyltransferase DOT1L controls spermatid chromatin remodelling and subsequent reorganization and compaction of spermatozoon genome. Using a mouse model in which Dot1l is knocked-out (KO) in postnatal male germ cells, we found that Dot1l-KO sperm chromatin is less compact and has an abnormal content, characterized by the presence of transition proteins, immature protamine 2 forms and a higher level of histones. Proteomics and transcriptomics analyses performed on spermatids reveal that Dot1l-KO modifies the chromatin prior to histone removal, and leads to the deregulation of genes involved in flagellum formation and apoptosis during spermatid differentiation. As a consequence of these chromatin and gene expression defects, Dot1l-KO spermatozoa have less compact heads and are less motile, which results in impaired fertility.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_11:00:53.616.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD030734
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Delphine PFLIEGER
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-05 02:23:23	ID requested
⏵ 1	2024-10-07 11:00:54	announced

10.6019/PXD030734;

submitter keyword: DOTL,mouse spermatogenesis

Yohann Couté
contact affiliation	Studying the Dynamics of Proteomes, Large-Scale Biology, CEA Grenoble
contact email	yohann.coute@cea.fr
lab head
Delphine PFLIEGER
contact affiliation	CNRS
contact email	delphine.pflieger@cea.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030734
     1. Label: PRIDE project
     2. Name: DOT1L regulates chromatin reorganization and gene expression during sperm differentiation