PXD030711-3

PXD030711 is an original dataset announced via ProteomeXchange.

Title	QuantitaQuantitative proteomic analysis of β-cells response to thapsigargin and rotenone uncovers key pathways of rat β-cell dysfunction tive proteomic analysis of β-cells response to thapsigargin and rotenone uncovers key pathways of rat β-cell dysfunction
Description	We designed a TMT peptide labeling-based quantitative proteomics strategy to achieve a high-accuracy analysis of proteomic changes in response to ER stress and mitochondria stress on INS-1 cells under three different culture conditions: control (Cont), rotenone (Rot), and thapsigargin (Tpg), each with five biological replicates. The TMT data was validated using label-free quantification method.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:56:13.009.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mehari Weldemariam
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-04 03:38:18	ID requested
1	2023-07-20 11:23:22	announced
2	2023-11-14 09:03:16	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:56:13	announced	2024-10-22: Updated project metadata.

10.1021/acs.chemrestox.2c00058;

Weldemariam MM, Woo J, Zhang Q, -Cell Dysfunction. Chem Res Toxicol, 35(6):1080-1094(2022) [pubmed]

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Diabetes (B/D-HPP)

submitter keyword: Endoplasmic reticulum (ER) stress

Mitochondria stress

Quantitative proteomics

TMTpro

INS-1 beta cells, cells

Metascape

Qibin Zhang
contact affiliation	Center for Translational Biomedical Research, University of North Carolina at Greensboro
contact email	q_zhang2@uncg.edu
lab head
Mehari Weldemariam
contact affiliation	The University of North Carolina at Greensboro
contact email	mmweldem@uncg.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD030711

PRIDE project URI

• PRIDE
  1. PXD030711
     1. Label: PRIDE project
     2. Name: QuantitaQuantitative proteomic analysis of β-cells response to thapsigargin and rotenone uncovers key pathways of rat β-cell dysfunction tive proteomic analysis of β-cells response to thapsigargin and rotenone uncovers key pathways of rat β-cell dysfunction