PXD030663-1

PXD030663 is an original dataset announced via ProteomeXchange.

Title	Cell-type-specific labeling and profiling of glycans in living mice
Description	Metabolic labeling of glycans with clickable unnatural sugars has enabled glycan imaging and glycoproteomics in mixed cell populations. However, in vivo labeling and profiling of cell-type-specific glycans remains challenging. Here, we develop genetically encoded metabolic glycan labeling (GeMGL), a cell-type-specific strategy based on a bump-hole pair of an unnatural sugar and its matching engineered enzyme. N pentynylacetylglucosamine (GlcNAl) serves as a bumped analog of N acetylglucosamine (GlcNAc) that can be specifically incorporated into glycans of cells expressing an engineered mutant of UDP-GlcNAc pyrophosphorylase, AGX2F383G. GeMGL with the 1,3-di-O-propionylated GlcNAl (1,3-Pr2GlcNAl) and AGX2F383G pair is demonstrated in cell co-cultures, and used for specific labeling of glycans in mouse xenograft tumors. By generating a transgenic mouse line with AGX2F383G expressed under cardiomyocyte-specific promoter, we perform specific imaging of cardiomyocyte glycans in the heart and identified 582 cardiomyocyte O-GlcNAcylated proteins with no interference from other cardiac cell types. GeMGL will facilitate cell-type-specific glycoproteomics in various tissues and disease models.
HostingRepository	PRIDE
AnnounceDate	2022-02-28
AnnouncementXML	Submission_2022-02-28_12:42:32.799.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qitao Song
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-(N-acetylamino)glucosyl-L-cysteine; O-(N-acetylamino)glucosyl-L-threonine; O-(N-acetylamino)glucosyl-L-serine
Instrument	Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-30 03:22:03	ID requested
⏵ 1	2022-02-28 12:42:33	announced

Dataset with its publication pending

submitter keyword: Glycan, cell-type-specific, metabolic glycan labeling

Xing Chen
contact affiliation	College of Chemistry and Molecular Engineering, Peking University, Beijing, China
contact email	xingchen@pku.edu.cn
lab head
Qitao Song
contact affiliation	Peking University
contact email	sqtwgq@sina.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030663
     1. Label: PRIDE project
     2. Name: Cell-type-specific labeling and profiling of glycans in living mice