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PXD030663 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCell-type-specific labeling and profiling of glycans in living mice
DescriptionMetabolic labeling of glycans with clickable unnatural sugars has enabled glycan imaging and glycoproteomics in mixed cell populations. However, in vivo labeling and profiling of cell-type-specific glycans remains challenging. Here, we develop genetically encoded metabolic glycan labeling (GeMGL), a cell-type-specific strategy based on a bump-hole pair of an unnatural sugar and its matching engineered enzyme. N pentynylacetylglucosamine (GlcNAl) serves as a bumped analog of N acetylglucosamine (GlcNAc) that can be specifically incorporated into glycans of cells expressing an engineered mutant of UDP-GlcNAc pyrophosphorylase, AGX2F383G. GeMGL with the 1,3-di-O-propionylated GlcNAl (1,3-Pr2GlcNAl) and AGX2F383G pair is demonstrated in cell co-cultures, and used for specific labeling of glycans in mouse xenograft tumors. By generating a transgenic mouse line with AGX2F383G expressed under cardiomyocyte-specific promoter, we perform specific imaging of cardiomyocyte glycans in the heart and identified 582 cardiomyocyte O-GlcNAcylated proteins with no interference from other cardiac cell types. GeMGL will facilitate cell-type-specific glycoproteomics in various tissues and disease models.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterQitao Song
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-(N-acetylamino)glucosyl-L-cysteine; O-(N-acetylamino)glucosyl-L-threonine; O-(N-acetylamino)glucosyl-L-serine
InstrumentQ Exactive; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-30 03:22:03ID requested
12022-02-28 12:42:33announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Glycan, cell-type-specific, metabolic glycan labeling
Contact List
Xing Chen
contact affiliationCollege of Chemistry and Molecular Engineering, Peking University, Beijing, China
contact emailxingchen@pku.edu.cn
lab head
Qitao Song
contact affiliationPeking University
contact emailsqtwgq@sina.com
dataset submitter
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Dataset FTP location
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