PXD030631 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | OXSR1 inhibits inflammasome activation by limiting potassium efflux during mycobacterial infection |
Description | Pathogenic mycobacteria inhibit inflammasome activation as part of their pathogenesis. While it is known that potassium efflux is a trigger for inflammasome activation, the interaction between mycobacterial infection, potassium efflux and inflammasome activation has not been investigated. Here we use Mycobacterium marinum infection of zebrafish embryos to demonstrate that pathogenic mycobacteria upregulate the host WNK signalling pathway kinases SPAK and OXSR1 which control intracellular potassium balance. We show that genetic depletion or inhibition of OXSR1 decreases bacterial burden and intracellular potassium levels. The protective effects of OXSR1 depletion are mediated by NLRP3 inflammasome activation and are dependent on caspase-mediated release of IL-1β and the downstream activation of protective TNF-α. The elucidation of this druggable pathway to potentiate inflammasome activation provides a new avenue for the development of host-directed therapies against intracellular infections. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-05 |
AnnouncementXML | Submission_2022-05-05_00:53:50.047.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | matthew O'Rourke |
SpeciesList | scientific name: Mycobacterium marinum; NCBI TaxID: 1781; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-12-24 00:35:09 | ID requested | |
⏵ 1 | 2022-05-05 00:53:50 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Human, Cell lines, LCMS, Mycobacterium |
Contact List
Stefan H Oehlers |
contact affiliation | 1. Tuberculosis Research Program Centenary Institute, The University of Sydney, Camperdown, NSW 2050, Australia 2. The University of Sydney, Discipline of Infectious Diseases & Immunology and Sydney Institute for Infectious Diseases, Camperdown, NSW 2050, Australia |
contact email | matthew.orourke@uts.edu.au |
lab head | |
matthew O'Rourke |
contact affiliation | University of Sydney |
contact email | matthew.orourke1@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD030631
- Label: PRIDE project
- Name: OXSR1 inhibits inflammasome activation by limiting potassium efflux during mycobacterial infection