PXD030630-1

PXD030630 is an original dataset announced via ProteomeXchange.

Title	Patterning of the autotrophic, mixotrophic and heterotrophic proteomes of oxygen evolving cyanobacterium Synechocystis sp. PCC 6803
Description	The trophic condition related proteomes of an oxygenic photosynthetic cyanobacterium Synechocystis sp. PCC 6803 (glucose tolerant strain) were analyzed to get further insights into the metabolic traits governing the early exponential growth under photoautotrophic, mixotrophic and heterotrophic conditions. The cells were grown under constant light autotrophically (AT) in low (air-level) CO2 (ATLC) and in high CO2 (ATHC), mixotrophically (MT) in low (air-level) CO2 in the presence of glucose (MTLC) and heterotrophically in low (air-level) CO2 in the presence of glucose and only 10 min exposure to light every 24 hours (Light-Activated Heterotrophic Growth; LAHGLC). The proteomes of ATHC, MTLC and LAHGLC differed substantially from that of ATLC, particularly by downregulation of the inducible carbon concentrating mechanisms (CCM) and photorespiration related enzymes. On the contrary, under carbon-rich conditions (ATHC, MTLC, LAHGLC) the cells accumulated respiratory NAD(P)H dehydrogenase I complexes in the thylakoid membrane. In glucose supplemented cultures (MTLC, LAHGLC) a distinct NDH-2 protein, NdbA, additionally accumulated in the thylakoid membrane whilst the plasma membrane localized NdbC and ARTO decreased in abundance. The light-harvesting proteins together with the photosystem I and II subunits were uniquely depleted under LAHGLC condition, accumulated under ATHC and in MTLC remained at the level of the reference ATLC condition. The accumulation of P-transporters was unique, revealing only minor differences between ATLC and ATHC but strong up-regulation in MTLC and down-regulation in LAHGLC. This likely implies that MTLC stores energy surplus in the highly energetic bonds of polyphosphate polymer that can be used under unfavorable growth conditions. It is concluded that the rigor of cell growth in MTLC condition results to a great extent from high intracellular inorganic carbon conditions created upon glucose breakdown and release of CO2, beside the direct utilization of glucose-derived carbon skeletons for growth. This combination provides the MTLC cultures with excellent conditions for growth that often exceeds that of the mere photoautotrophic growth at high CO2.
HostingRepository	PRIDE
AnnounceDate	2022-04-13
AnnouncementXML	Submission_2022-04-13_05:47:38.250.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD030630
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dorota Muth-Pawlak
SpeciesList	scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-24 00:32:42	ID requested
⏵ 1	2022-04-13 05:47:38	announced

Dataset with its publication pending

submitter keyword: Synechocystis, autotrophy, heterotrophy, mixotrophy, photosynthesis, carbon metabolism, adaptation

Eva-Mari Aro
contact affiliation	University of Turku Faculty of Technology Department of Life Technologies Molecular Plant Biology Group Tykistökatu 6 20520 Turku Finland
contact email	evaaro@utu.fi
lab head
Dorota Muth-Pawlak
contact affiliation	University of Turku
contact email	dokrmu@utu.fi
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030630
     1. Label: PRIDE project
     2. Name: Patterning of the autotrophic, mixotrophic and heterotrophic proteomes of oxygen evolving cyanobacterium Synechocystis sp. PCC 6803