PXD030615-1

PXD030615 is an original dataset announced via ProteomeXchange.

Title	Interfering with aggregated α-synuclein in advanced melanoma leads to a major upregulation of MHC class II proteins
Description	Melanoma is the most serious and deadly form of skin cancer and with progression to advanced melanoma, the intrinsically disordered protein α-synuclein is upregulated to high levels; and while toxic to dopaminergic neurons in Parkinson’s disease, it is highly beneficial for primary and metastatic melanoma cells. To gain detailed insights, both at the level of the proteome and the transcriptome, into this exact opposite role of α-synuclein in advanced melanoma, we performed proteome and transcriptome studies of high-level α-synuclein-expressing primary and metastatic human melanoma cell lines and metastatic human melanoma xenografts treated with the small-molecule diphenyl-pyrazole compound anle138b, which binds to and interferes with the oligomeric structure of α-synuclein. Our data demonstrate that interfering with oligomerized α-synuclein in these melanoma cells and tumor xenografts leads to a substantial upregulation of major histocompatibility complex (MHC) proteins, which are important for enhancing anti-melanoma immune responses.
HostingRepository	PRIDE
AnnounceDate	2024-07-04
AnnouncementXML	Submission_2024-07-03_16:38:57.078.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ivan Silbern
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion Lumos; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-23 00:22:51	ID requested
⏵ 1	2024-07-03 16:38:57	announced

10.1097/CMR.0000000000000982;

submitter keyword: MHC, xenograft,melanoma, anle138b

Prof. Dr. Henning Urlaub
contact affiliation	Institute for Biophysical Chemistry, Bioanalytical Mass Spectrometry group, Am Fassberg 11, 37077, Goettingen
contact email	henning.urlaub@mpibpc.mpg.de
lab head
Ivan Silbern
contact affiliation	MPI Biophysical Chemistry
contact email	ivansilbern@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030615
     1. Label: PRIDE project
     2. Name: Interfering with aggregated α-synuclein in advanced melanoma leads to a major upregulation of MHC class II proteins