PXD030604-1

PXD030604 is an original dataset announced via ProteomeXchange.

Title	Complexome profiling to study consequences of membrane protein complexes in cells with knock down of ceramide synthase 4 and 5 in HCT-116 and Caco-2 cells.
Description	Ceramide synthases are central enzymes of the sphingolipid pathway and are important for the production of sphingolipids of different chain length. Sphingolipids of different chain length impact the physicochemical properties of membranes. Here we investigated by complexome profiling how the expression level of membrane proteins is affected in human colon cancer cells (Caco-2 and HCT-116) that were transduced with a shRNA against CerS4 or CerS5 to downregulate both enzymes.
HostingRepository	PRIDE
AnnounceDate	2025-01-07
AnnouncementXML	Submission_2025-01-07_06:14:50.779.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilka Wittig
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-22 07:34:04	ID requested
⏵ 1	2025-01-07 06:14:51	announced

El Hindi K, Brachtendorf S, Hartel JC, Renn, é C, Birod K, Schilling K, Labocha S, Thomas D, Ferreir, ó, s N, Hahnefeld L, Dorochow E, Del Turco D, Deller T, Scholich K, Fuhrmann DC, Weigert A, Br, ü, ne B, Geisslinger G, Wittig I, Link KH, Gr, ö, sch S, Hypoxia induced deregulation of sphingolipids in colon cancer is a prognostic marker for patient outcome. Biochim Biophys Acta Mol Basis Dis, 1870(1):166906(2024) [pubmed]

10.1016/j.bbadis.2023.166906;

submitter keyword: LC-MSMS, complexome profiling

Sabine Grösch
contact affiliation	Institute of Clinical Pharmacology, Goethe-University, Frankfurt, Germany
contact email	groesch@em.uni-frankfurt.de
lab head
Ilka Wittig
contact affiliation	Functional Proteomics, Goethe University, Frankfurt am Main , Germany
contact email	wittig@med.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD030604

PRIDE project URI

• PRIDE
  1. PXD030604
     1. Label: PRIDE project
     2. Name: Complexome profiling to study consequences of membrane protein complexes in cells with knock down of ceramide synthase 4 and 5 in HCT-116 and Caco-2 cells.