PXD030572-2

PXD030572 is an original dataset announced via ProteomeXchange.

Title	Time-course proteomic analysis of irradiated human endothelial cells
Description	The vascular endothelium is a hot spot in the response to radiation therapy for both tumors and normal tissues. To improve patient outcomes, interpretable systemic hypotheses are needed to help radiobiologists and radiation oncologists propose endothelial targets that could protect normal tissues from the adverse effects of radiation therapy and/or enhance its antitumor potential. To this end, we captured the kinetics of multi-omics layers – i.e. miRNome, targeted transcriptome, proteome and metabolome – in irradiated primary human endothelial cells cultured in vitro. We then designed a strategy of deep learning as in convolutional graph networks that facilitates unsupervised high-level feature extraction of important omics data to learn how ionizing radiation-induced endothelial dysfunction may evolve over time. Last, we present experimental data showing that some of the features identified using our approach are involved in the alteration of angiogenesis by ionizing radiation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:30:54.558.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olivier Guipaud
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue
Instrument	6520A Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-21 08:15:31	ID requested
1	2024-10-08 11:37:38	announced
⏵ 2	2024-10-22 05:30:54	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Human, LC-MSMS, ionising radiation, Endothelial cells

Fabien Milliat
contact affiliation	PSE-SANTE/SERAMED, LRMED, IRSN
contact email	fabien.milliat@irsn.fr
lab head
Olivier Guipaud
contact affiliation	IRSN
contact email	olivier.guipaud@irsn.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD030572

PRIDE project URI

• PRIDE
  1. PXD030572
     1. Label: PRIDE project
     2. Name: Time-course proteomic analysis of irradiated human endothelial cells