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PXD030552-1

PXD030552 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCharacterization of progressive denaturation and unfolding intermediates of proteins by fast cross-linking using di-ortho-phthalaldehyde
DescriptionChemical cross-linking of proteins coupled with mass spectrometry (CXMS) is widely used in protein structural analysis. In this study we develop a new class of non-hydrolyzable amine-selective di-ortho-phthalaldehyde (DOPA) cross-linkers, one of which is DOPA2. Cross-linking of proteins with DOPA2 is 60-120 times faster than that with the N-hydroxysuccinimide ester cross-linker DSS. Compared with DSS cross-links, DOPA2 cross-links have a higher degree of agreement with the crystal structures of tested proteins. More importantly, DOPA2 has unique advantages of working at low pH, low temperature, or in the presence of denaturants such as 8 M urea or 6 M guanidine hydrochloride. Using staphylococcal nuclease, bovine serum albumin, and bovine pancreatic ribonuclease A, we demonstrate that DOPA2 cross-linking provides abundant spatial information about the conformations of proteins denatured to varying degrees. Lastly, we show that CXMS with only 10 seconds of DOPA cross-linking uncovers conformational changes ass
HostingRepositoryiProX
AnnounceDate2021-12-20
AnnouncementXMLSubmission_2021-12-20_20:04:03.616.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJian-Hua Wang
SpeciesList scientific name: Staphylococcus aureus; NCBI TaxID: 1280; scientific name: Bos taurus; NCBI TaxID: 9913;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-20 20:03:36ID requested
12021-12-20 20:04:03announced
22022-03-20 20:52:48announced2022-03-21: Update publication information.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: CXMS, Amine-selective cross-linker, Protein unfolding, LC-MS/MS
Contact List
Meng-Qiu Dong
contact affiliationNational Institute of Biological Sciences, Beijing
contact emaildongmengqiu@nibs.ac.cn
lab head
Jian-Hua Wang
contact affiliationNational Institute of Biological Sciences, Beijing
contact emailwangjianhua@nibs.ac.cn
dataset submitter
Full Dataset Link List
iProX dataset URI