PXD030552-1
PXD030552 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Characterization of progressive denaturation and unfolding intermediates of proteins by fast cross-linking using di-ortho-phthalaldehyde |
Description | Chemical cross-linking of proteins coupled with mass spectrometry (CXMS) is widely used in protein structural analysis. In this study we develop a new class of non-hydrolyzable amine-selective di-ortho-phthalaldehyde (DOPA) cross-linkers, one of which is DOPA2. Cross-linking of proteins with DOPA2 is 60-120 times faster than that with the N-hydroxysuccinimide ester cross-linker DSS. Compared with DSS cross-links, DOPA2 cross-links have a higher degree of agreement with the crystal structures of tested proteins. More importantly, DOPA2 has unique advantages of working at low pH, low temperature, or in the presence of denaturants such as 8 M urea or 6 M guanidine hydrochloride. Using staphylococcal nuclease, bovine serum albumin, and bovine pancreatic ribonuclease A, we demonstrate that DOPA2 cross-linking provides abundant spatial information about the conformations of proteins denatured to varying degrees. Lastly, we show that CXMS with only 10 seconds of DOPA cross-linking uncovers conformational changes ass |
HostingRepository | iProX |
AnnounceDate | 2021-12-20 |
AnnouncementXML | Submission_2021-12-20_20:04:03.616.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jian-Hua Wang |
SpeciesList | scientific name: Staphylococcus aureus; NCBI TaxID: 1280; scientific name: Bos taurus; NCBI TaxID: 9913; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-12-20 20:03:36 | ID requested | |
⏵ 1 | 2021-12-20 20:04:03 | announced | |
2 | 2022-03-20 20:52:48 | announced | 2022-03-21: Update publication information. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: CXMS, Amine-selective cross-linker, Protein unfolding, LC-MS/MS |
Contact List
Meng-Qiu Dong | |
---|---|
contact affiliation | National Institute of Biological Sciences, Beijing |
contact email | dongmengqiu@nibs.ac.cn |
lab head | |
Jian-Hua Wang | |
contact affiliation | National Institute of Biological Sciences, Beijing |
contact email | wangjianhua@nibs.ac.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |