PXD030509-1

PXD030509 is an original dataset announced via ProteomeXchange.

Title	A peptide-derived strategy for specifically targeting the mitochondrial-ER network of cancer cells: a new paradigm in fighting cancer
Description	An effective anti-cancer therapy should exclusively target cancer cells and trigger in them a broad spectrum of cell death pathways that will prevent avoidance. Here, we present a novel paradigm in cancer therapy that specifically targets the mitochondria and ER of cancer cells. We developed a peptide derived from the flexible and transmembrane domains of the human protein NAF-1/CISD2. This peptide (NAF-1) specifically permeated through the plasma membranes of human epithelial breast cancer cells, abolished their mitochondrial-ER network, and triggered cell death with characteristics of apoptosis, ferroptosis and necroptosis. In vivo analysis revealed that the peptide significantly decreased tumor growth in mice carrying xenograft human tumors. Computational simulations of cancer vs. normal cells membranes revealed that the specificity of the peptide to cancer cells is due to its selective recognition of their membrane composition. NAF-1 represents therefore a promising anti-cancer lead compound that acts via a unique mechanism.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_08:59:55.182.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pei Liu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-17 14:01:07	ID requested
⏵ 1	2022-08-12 08:59:55	announced
2	2024-10-22 05:39:07	announced	2024-10-22: Updated project metadata.

Sohn YS, Losub-Amir A, Cardenas AE, Karmi O, Yahana MD, Gruman T, Rowland L, Marjault HB, Webb LJ, Mittler R, Elber R, Friedler A, Nechushtai R, A peptide-derived strategy for specifically targeting the mitochondria and ER of cancer cells: a new approach in fighting cancer. Chem Sci, 13(23):6929-6941(2022) [pubmed]

submitter keyword: NAF-1, CISD2, cancer, cell death, mitochondria, ER, peptides

Ron Mittler
contact affiliation	Department of Surgery, University of Missouri School of Medicine, Christopher S. Bond Life Sciences Center University of Missouri. 1201 Rollins St, Columbia, MO 65201, USA The Division of Plant Sciences and Interdisciplinary Plant Group, College of Agriculture, Food and Natural Resources, Christopher S. Bond Life Sciences Center University of Missouri. 1201 Rollins St, Columbia, MO, 65201, USA.
contact email	mittlerr@missouri.edu
lab head
Pei Liu
contact affiliation	University of Missouri, columbia
contact email	liup1@missouri.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD030509

PRIDE project URI

• PRIDE
  1. PXD030509
     1. Label: PRIDE project
     2. Name: A peptide-derived strategy for specifically targeting the mitochondrial-ER network of cancer cells: a new paradigm in fighting cancer