<<< Full experiment listing

PXD030505-1

PXD030505 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAnalysis of unfolded protein response activation in colon adenocarcinoma epithelial cells: a proteomic study
DescriptionMolecular patterns in colorectal cancer (CRC) cells have allowed to model responses to anti-cancer treatments. The different responses observed depend on the type of cancer, the tumor grade and the functional programme of the cancer cells. Recent studies suggest that the unfolded protein response (UPR), the autophagy and the apoptosis could be involved in treatment resistance mechanisms by interacting with the tumor microenvironment (TME). In this study, we analysed by LC-MS/MS the proteome of two representative colon adenocarcinoma epithelial cell lines from different tumor grade (CCL-233 and CCL-221) at basal state or after the UPR induction. Results showed that cell lines expressed a different proteome on about 10% of their total proteins identified, and especially on UPR, autophagy and apoptosis pathways proteins at basal state. After UPR induction, the proteome of the cells was modified with a greater adaptive response to cellular stress in CCL-221 cells where the UPR was strongly activated at basal state. Thus, we demonstrated that CRC cell lines at different tumor grades did not express the same functional programme at the proteomic level and were characterized by different responses to the UPR induction. This study suggests that baseline cancer cell stress status could have an impact on the efficiency of cancer therapies.
HostingRepositoryPRIDE
AnnounceDate2024-09-04
AnnouncementXMLSubmission_2024-09-04_01:11:16.220.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBRAY FABRICE
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-17 13:50:55ID requested
12024-09-04 01:11:16announced
22024-10-22 06:57:35announced2024-10-22: Updated project metadata.
Publication List
10.1002/PRCA.202400008;
Keyword List
submitter keyword: Human, CCL-211, proteomics, fibroblast, CL-233, quantification
Contact List
Fabrice bray
contact affiliationMSAP UAR 3290 CNRS
contact emailfabrice.bray@univ-lille.fr
lab head
BRAY FABRICE
contact affiliationMSAP USR 3290
contact emailfabrice.bray@univ-lille.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD030505
PRIDE project URI
Repository Record List
[ + ]