PXD030497-1

PXD030497 is an original dataset announced via ProteomeXchange.

Title	MS-based quantitative proteomic analysis of embryonic stem cells with normal and reduced expression of Rsl24d1.
Description	Embryonic stem cell (ESC) fate decisions are regulated by a complex molecular circuitry that requires tight and coordinated gene expression regulations at multiple levels from chromatin organization to mRNA processing. Recently, ribosome biogenesis and translation have emerged as key pathways that efficiently control stem cell homeostasis. However, the molecular mechanisms underlying the regulation of these pathways remain largely unknown to date. Here, we analyzed the expression, in mouse ESCs, of over 300 genes involved in ribosome biogenesis and we identified RSL24D1 as the most differentially expressed between self-renewing and differentiated ESCs. RSL24D1 is highly expressed in multiple mouse pluripotent stem cell models and its expression profile is conserved in human ESCs. RSL24D1 is associated with nuclear pre-ribosomes and is required for the maturation and the synthesis of 60S subunits in mouse ESCs. Interestingly, RSL24D1 depletion significantly impairs global translation, particularly of key pluripotency factors, including POU5F1 and NANOG, as well as components of the polycomb repressive complex 2 (PRC2). Consistently, RSL24D1 is required for mouse ESC self-renewal and proliferation. Taken together, we show that RSL24D1-dependant ribosome biogenesis is required to both sustain the expression of pluripotent transcriptional programs and silence developmental programs, which concertedly dictate ESC homeostasis.
HostingRepository	PRIDE
AnnounceDate	2023-01-10
AnnouncementXML	Submission_2023-01-10_02:28:31.071.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	YohannCouté
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-17 13:27:30	ID requested
⏵ 1	2023-01-10 02:28:31	announced
2	2023-01-26 02:03:57	announced	2023-01-26: Updated project metadata.
3	2023-11-14 07:28:37	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: translational program, pluripotency,Embryonic stem cell, ribosome biogenesis, nanoLC-MS/MS

YohannCouté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
lab head
YohannCouté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030497
     1. Label: PRIDE project
     2. Name: MS-based quantitative proteomic analysis of embryonic stem cells with normal and reduced expression of Rsl24d1.