PXD030486-2

PXD030486 is an original dataset announced via ProteomeXchange.

Title	A specialized Hsp90 co-chaperone network regulates steroid hormone receptor response to ligand
Description	Heat shock protein-90 chaperone machinery is involved in the stability and activity of its client proteins. The chaperone function of Hsp90 is regulated by co-chaperones and post-translational modifications. Although structural evidence exists for Hsp90 interaction with clients, our understanding of the impact of Hsp90 chaperone function towards client activity in cells remains elusive. Here, we dissected the impact of newly identified co-chaperones in higher eukaryotes, FNIP1/2 (FNIPs) and Tsc1, towards Hsp90 client activity. Our data show that Tsc1 and FNIP2 form mutually exclusive complexes with FNIP1 and that unlike Tsc1, FNIP1/2 interact with the catalytic residue of Hsp90. Functionally, these co-chaperone complexes increase the affinity of the steroid hormone receptors glucocorticoid receptor and estrogen receptor to their ligands in vivo. Here, we provide a model for the responsiveness of the steroid hormone receptor activation upon ligand binding as a consequence of their association with specific Hsp90:co-chaperone subpopulations.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:45:56.069.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sarah Backe
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	5800 TOF/TOF

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-17 04:56:30	ID requested
1	2024-10-08 11:38:04	announced
⏵ 2	2024-10-22 05:45:56	announced	2024-10-22: Updated project metadata.

10.1016/J.CELREP.2022.111039;

submitter keyword: Hsp90, Co-Chaperone, Chaperone, Glucocorticoid receptor, Tsc1, FNIP1, FNIP2, Steroid hormone receptors

Mehdi Mollapour
contact affiliation	Department of Urology, SUNY Upstate Medical University Syracuse, NY 13210, USA
contact email	mollapom@upstate.edu
lab head
Sarah Backe
contact affiliation	SUNY Upstate Medical University
contact email	backes@upstate.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD030486

PRIDE project URI

• PRIDE
  1. PXD030486
     1. Label: PRIDE project
     2. Name: A specialized Hsp90 co-chaperone network regulates steroid hormone receptor response to ligand