PXD030467-1

PXD030467 is an original dataset announced via ProteomeXchange.

Title	Functional Requirements for a Samd14-Capping Protein Complex in Stress Erythropoiesis
Description	Acute anemia induces rapid expansion of erythroid precursors and accelerated differentiation to replenish erythrocytes. Paracrine signals – involving cooperation between SCF/c-Kit signaling and other signaling inputs – are required for the activation and function of erythroid precursors in anemia. Our prior work revealed that the Sterile Alpha Motif (SAM) Domain 14 (Samd14) gene is transcriptionally upregulated in a model of acute anemia. Samd14 expression increases the regenerative capacity of the erythroid system and promotes stress-dependent c-Kit signaling. However, the mechanism underlying Samd14’s role in stress erythropoiesis is unknown. We identified a protein-protein interaction between Samd14 and the α- and β heterodimers of the F-actin capping protein (CP) complex. Knockdown of the CP β subunit increased erythroid maturation in ex vivo cultures and decreased colony forming potential of stress erythroid precursors. In a genetic complementation assay for Samd14 activity, our results revealed that the Samd14-CP interaction is a determinant of erythroid precursor cell levels and function. Samd14-CP promotes SCF/c-kit signaling in CD71med spleen erythroid precursors.
HostingRepository	PRIDE
AnnounceDate	2022-08-09
AnnouncementXML	Submission_2022-08-09_07:51:03.167.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD030467
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sophe Alvarez
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-16 12:33:55	ID requested
⏵ 1	2022-08-09 07:51:03	announced

Dataset with its publication pending

submitter keyword: Samd14

capping protein

c-Kit

erythropoietin

stress erythropoiesis

Sophie Alvarez
contact affiliation	Proteomics and Metabolomics Facility, University of Nebraska- Lincoln
contact email	salvarez@unl.edu
lab head
Sophe Alvarez
contact affiliation	University of Nebraska - Lincoln
contact email	salvarez@unl.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030467
     1. Label: PRIDE project
     2. Name: Functional Requirements for a Samd14-Capping Protein Complex in Stress Erythropoiesis