PXD030403-1

PXD030403 is an original dataset announced via ProteomeXchange.

Title	Thyroid Hormone Dependent Transcriptional Programming by TRb Requires SWI/SNF Chromatin Remodelers
Description	Transcriptional regulation in response to thyroid hormone (3,5,3´-triiodo-L-thyronine, T3) is a dynamic and cell-type specific process that maintains cellular homeostasis and identity in all tissues. However, our understanding of the mechanisms of thyroid hormone receptor (TR) actions at the molecular level are actively being refined. We used an integrated genomics approach to profile and characterize the cistrome of TRb, map changes in chromatin accessibility, and capture the transcriptomic changes in response to T3 in normal human thyroid cells. There are significant shifts in TRb genomic occupancy in response to T3, which are associated with differential chromatin accessibility, and differential recruitment of SWI/SNF chromatin remodelers. We further demonstrate selective recruitment of BAF and PBAF SWI/SNF complexes to TRb binding sites, revealing novel differential functions in regulating chromatin accessibility and gene expression. Our findings highlight three distinct modes of TRb interaction with chromatin and coordination of coregulator activity.
HostingRepository	PRIDE
AnnounceDate	2022-05-25
AnnouncementXML	Submission_2022-05-25_04:56:06.509.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Noelle Gillis
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-14 09:55:13	ID requested
⏵ 1	2022-05-25 04:56:07	announced

Gillis NE, Boyd JR, Tomczak JA, Frietze S, Carr FE, requires SWI/SNF chromatin remodelers. Nucleic Acids Res, 50(3):1382-1395(2022) [pubmed]

submitter keyword: thyroid hormone receptor, transcription, coregulators, nuclear receptor

Frances Carr
contact affiliation	Department of Pharmacology, University of Vermont Larner College of Medicine
contact email	fcarr@uvm.edu
lab head
Noelle Gillis
contact affiliation	University of Vermont
contact email	negillis@uvm.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030403
     1. Label: PRIDE project
     2. Name: Thyroid Hormone Dependent Transcriptional Programming by TRb Requires SWI/SNF Chromatin Remodelers