PXD030402-1

PXD030402 is an original dataset announced via ProteomeXchange.

Title	SILAC APMS analysis of GFP tagged TSC2 in Hela Cells
Description	HSP90 and its co-chaperon R2TP are involved to assemble and stabilize many macro-molecular complexes involved in cell proliferation. R2TP complex is composed of RUVBL1 and RUVBL2 AAA+ ATPases and two adapter proteins RPAP3 and PIH1D1. The N-terminal domain of PIH1D1 was described to possess a basic pocket that binds phosphorylated peptides on some of the R2TP clients. Subunits of TSC complex (Tuberous SClerosis) have previously been described in proteomic analysis of proteins immune-precipitated with PIH1D1 N-terminal domain. TSC complex is a regulator of mTOR activity. It is composed of TSC1, TSC2 and TBC1D7 subunits. Here, we show that (i) TSC1 and TSC2 are both substrates of HSP90, (ii) the HSP90/R2TP system has multiple interaction with TSC complex subunits and (iii) it is functionally involved in the assembly of the TSC complex. We found a direct interaction of TSC1 with the phospho-binding pocket of PIH1D1 N-terminal domain regardless of phosphorylationbut in a non-canonical phosphorylation independent manner[XM1] . TSC2 and TBC1D7 would also interact with R2TP but independently of PIH1D1. Taken together, these data suggest that R2TP acts as a platform for the independent recruitment of TSC subunits before the assembly of the TSC complex
HostingRepository	PRIDE
AnnounceDate	2024-11-12
AnnouncementXML	Submission_2024-11-12_01:09:26.138.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Franck Vandermoere
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-14 09:54:03	ID requested
⏵ 1	2024-11-12 01:09:26	announced

10.1016/J.JMB.2024.168840;

submitter keyword: immunoprecipitation,SILAC, GFP

Celine Verheggen
contact affiliation	IGH, CNRS, Univ Montpellier, Montpellier, France
contact email	celine.verheggen@igh.cnrs.fr
lab head
Franck Vandermoere
contact affiliation	IGF, CNRS, INSERM, University of Montpellier, Montpellier, France
contact email	franck.vandermoere@igf.cnrs.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030402
     1. Label: PRIDE project
     2. Name: SILAC APMS analysis of GFP tagged TSC2 in Hela Cells