PXD030386 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Nogo-A modulates gene expression to fine-tune enamel development |
Description | Nogo-A is a major player in neural development and regeneration, and it is the target of several clinical trials. However, its functions outside the nervous system are mostly unknown. We observed that Nogo-A is expressed in dental epithelial cells, responsible for the formation of enamel, and we showed that the deletion of Nogo-A in transgenic mouse models leads to the formation of defective enamel. We observed that Nogo-A directly interacts with molecules important for gene expression regulation, and its deletion perturbs their cellular localization. As a result, Nogo-A deletion induces overexpression of genes involved in cell differentiation and enamel production. Mechanistically, we demonstrated that intracellular Nogo-A, and not cell surface Nogo-A, is responsible for gene expression modulation. Taken together, our results indicate a new role for Nogo-A as regulator of enamel formation and suggest a new possible cell-autonomous function in regulating gene expression and cell differentiation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-06-20 |
AnnouncementXML | Submission_2024-06-19_23:56:46.586.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Witold Wolski |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-12-14 06:58:45 | ID requested | |
⏵ 1 | 2024-06-19 23:56:46 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Differentiation, Amelogenesis, LC-MS, Tooth, Interactome,Nogo-A, Development, Enamel, Transcriptome, DDA |
Contact List
Pierfrancesco Pagella |
contact affiliation | Institute of Oral Biology Center of Dental Medicine (ZZM) University of Zurich (UZH) |
contact email | pierfrancesco.pagella@uzh.ch |
lab head | |
Witold Wolski |
contact affiliation | Functional Genomics Center Zurch |
contact email | wew@fgcz.ethz.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030386
- Label: PRIDE project
- Name: Nogo-A modulates gene expression to fine-tune enamel development