PXD030364-1

PXD030364 is an original dataset announced via ProteomeXchange.

Title	Quantification of parental and new histones H3, H4 and H2A by mass spectrometry: analysis of their dynamics along cell cycle progression in WT and MCM2-2A mutant mouse ESCs
Description	During DNA replication parental, nucleosomic histones are segregated almost symmetrically to the two daughter DNA strands enabling mitotic inheritance of histones and their PTMs. In MCM2 histone-binding mutant ESCs (MCM2-2A), histones are recycled asymmetrically to the leading strand. To evaluate if loss of parental histones contributes to the asymmetry, we tracked new and old histones quantitatively by pulse-Triple-SILAC-MS. The quantification showed no change in dilution and incorporation dynamics in MCM2-2A cells, demonstrating that parental histones are not lost at replication forks but re-routed from the lagging to the leading strand.
HostingRepository	PRIDE
AnnounceDate	2023-08-30
AnnouncementXML	Submission_2023-08-30_04:44:53.725.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	VictorSolis
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	N6-propanoyl-L-lysine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-13 07:09:19	ID requested
⏵ 1	2023-08-30 04:44:54	announced
2	2023-11-14 08:10:37	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:02:15	announced	2024-10-22: Updated project metadata.

submitter keyword: histone,Mouse, MS, SILAC, pulse-SILAC, ESC, MCM2

AnjaGroh
contact affiliation	Novo Nordisk Foundation Center for Protein Research (CPR), University of Copenhagen, Copenhagen, Denmark.
contact email	anja.groth@cpr.ku.dk
lab head
VictorSolis
contact affiliation	EpiQMAx GmbH
contact email	solis@genzentrum.lmu.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030364
     1. Label: PRIDE project
     2. Name: Quantification of parental and new histones H3, H4 and H2A by mass spectrometry: analysis of their dynamics along cell cycle progression in WT and MCM2-2A mutant mouse ESCs