PXD030275-1

PXD030275 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomics Revealed the Changes of Pharmacodynamic Network of Bugu Shengsui Decoction Promoting Osteoblasts Proliferation
Description	Insufficient osteogenesis is greatly essential to osteoporosis. Bugu Shengsui Decoction, a compound formula for osteoporosis, has significant clinical effects in the treatment of osteoporosis. Yet the detailed mechanisms are unclear. In this study, we first evaluated the pharmacological effects of Bugu Shengsui Decoction on bone metabolism, bone mineral density, bone morphology and biomechanics in ovariectomized rats. It also clarified the promoting effect of Bugu Shengsui Decoction on the proliferation and differentiation of osteoblasts. Based on the above results, we conducted quantitative proteomics research and a series of validation experiments, the results showed that PI3K-AKT signaling pathway and targets, such as Fn1, Col1a1, Col1a2, Col6a1, Col6a2, Col6a3, Rac1, Hsp90ab1, Hsp90b1, Ywhaz and Gnb2, were most relevant to the anti-osteoporosis efficacy of BGSSD. Summarily, our discoveries certify that Bugu Shengsui Decoction is an effective treatment for osteoporosis via PI3K-AKT.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:44:04.601.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Baoyu Qi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	2x(13)C,6x(2)H labeled dimethylated L-arginine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-09 02:59:10	ID requested
⏵ 1	2024-10-07 13:44:05	announced

Dataset with its publication pending

submitter keyword: Osteoporosis, Osteoblasts, Bugu Shengsui Decoction, Proliferation, Quantitative Proteomics, PI3K-Akt, Pharmacodynamic Network

Jianye Dai
contact affiliation	School of Pharmacy, Lanzhou University, Lanzhou, China
contact email	daijy@lzu.edu.cn
lab head
Baoyu Qi
contact affiliation	Wangjing Hospital, China Academy of Chinese Medical Sciences
contact email	qiby325@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030275
     1. Label: PRIDE project
     2. Name: Quantitative Proteomics Revealed the Changes of Pharmacodynamic Network of Bugu Shengsui Decoction Promoting Osteoblasts Proliferation