PXD030260-1

PXD030260 is an original dataset announced via ProteomeXchange.

Title	Plasma proteomic, human Ebola virus disease
Description	The 2013-2016 Ebola Zaire virus (EBV) outbreak in West Africa resulted in over 28,000 cases and 11,000 deaths. Ebola virus disease (EVD) is a highly virulent systemic disease with a high case fatality rate of ~ 50%. EVD results in hemorrhagic fever marked by an exaggerated systemic inflammatory response, and impaired vascular and coagulation systems. The immune response of patients who either survived or died is characterized by strong differences. Notably, fatalities showed a diminished capacity to mount an appropriate immune response, resulting in high viremia and increased pro-inflammatory cytokine production. In this study, we analyzed 38 sequential samples collected from 12 patients: 8 survivors and 4 fatalities. Our analytical strategy combined three protein-based platforms covering three different fractions of the plasma proteome: the undepleted classical plasma proteome, the depleted plasma proteome, and cytokines/chemokines, using LC/MS- and antibody-based assays, resulting in over 1000 quantified host and pathogen proteins. For depletion of the most abundant plasma proteins, we advanced a perchloric acid-based precipitation method. This method is low cost, high-throughput and robust.
HostingRepository	PRIDE
AnnounceDate	2022-03-29
AnnouncementXML	Submission_2022-03-29_14:06:40.082.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Arthur VIODE
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-08 12:42:21	ID requested
⏵ 1	2022-03-29 14:06:40	announced
2	2024-10-22 05:35:37	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: human,plasma,Ebola virus disease

Hanno Steen
contact affiliation	Department of Pathology, Boston Children’s Hospital, Boston, MA, USA Harvard Medical School, Boston, MA, USA Precision Vaccines Program, Boston Children’s Hospital, Boston, MA, USA
contact email	hanno.steen@childrens.harvard.edu
lab head
Arthur VIODE
contact affiliation	Department of Pathology, Boston Children’s Hospital, Boston, MA, USA Harvard Medical School, Boston, MA, USA
contact email	arthur.viode@childrens.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD030260

PRIDE project URI

• PRIDE
  1. PXD030260
     1. Label: PRIDE project
     2. Name: Plasma proteomic, human Ebola virus disease