PXD030199-3

PXD030199 is an original dataset announced via ProteomeXchange.

Title	Complement System Activation Is a Plasma Biomarker Signature during Malaria in Pregnancy
Description	Malaria in pregnancy (MiP) is a public health problem in malaria-endemic areas, contributing to detrimental outcomes for both mother and fetus. Primigravida and second-time mothers are most affected by severe anaemia complications and babies with low birth weight compared to multi-gravida women. Infected erythrocytes (IE) reach the placenta, activating the immune response by placental monocyte infiltration and inflammation. However, specific markers of MiP result in poor outcomes, such as low birth weight, and intrauterine growth restriction for babies and maternal anaemia in women infected with Plasmodium falciparum are limited. In this study, we identified the plasma proteome signature of a mouse model infected with Plasmodium berghei ANKA and pregnant women infected with Plasmodium falciparum infection using quantitative mass spec-trometry-based proteomics. A total of 279 and 249 proteins were quantified in murine and human plasma samples, of which 28% and 30% were regulated proteins, respectively. Most of the regulated proteins in both organisms are involved in complement system activation during malaria in pregnancy. CBA anaphylatoxin assay confirmed the complement system activation by the increase in C3a and C4a anaphylatoxins in the infected plasma compared to non-infected plasma. Moreover, correlation analysis showed the association between complement system activation and reduced head circumference in newborns from Pf-infected mothers. The data obtained in this study highlight the correlation between the complement system and immune and newborn outcomes resulting from malaria in pregnancy.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:08:34.879.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Veronica Santiago
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-06 02:34:20	ID requested
1	2023-10-24 11:06:50	announced
2	2023-11-14 09:07:24	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:08:35	announced	2024-10-22: Updated project metadata.

10.3390/genes14081624;

Santiago VF, Dombrowski JG, Kawahara R, Rosa-Fernandes L, Mule SN, Murillo O, Santana TV, Coutinho JVP, Macedo-da-Silva J, Lazari LC, Peixoto EPM, Ramirez MI, Larsen MR, Marinho CRF, Palmisano G, Complement System Activation Is a Plasma Biomarker Signature during Malaria in Pregnancy. Genes (Basel), 14(8):(2023) [pubmed]

submitter keyword: proteomics, complement system, malaria in pregnancy,protein signature

Giuseppe Palmisano
contact affiliation	Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
contact email	palmisano.gp@usp.br
lab head
Veronica Santiago
contact affiliation	University of Southampton
contact email	veronicafeijoli@usp.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD030199

PRIDE project URI

• PRIDE
  1. PXD030199
     1. Label: PRIDE project
     2. Name: Complement System Activation Is a Plasma Biomarker Signature during Malaria in Pregnancy