PXD030183-1

PXD030183 is an original dataset announced via ProteomeXchange.

Title	A Bifunctional SPD-0090 contributes virulence of Streptococcus pneumoniae by affecting iron transport and galactose metabolism
Description	Streptococcus pneumoniae (S.pneumoniae) is a Gram-positive bacterial pathogen that colonizes on the mucosal surfaces of the host's nasopharynx and upper respiratory tract and results in pneumonia. In order to survive and infect the host, S.pneumoniae must have obtain essential nutrients, such as transition metal ions . Our previous study had shown that the mRNA and protein levels of SPD-0090 are significantly upregulated in the ΔpiuA/ΔpiaA/ΔpitA triple mutant (three major iron transports), but its detailed biological function is unknown. In this study, we found that the knockout spd-0090 gene induced a delayed growth in the medium with different sugar sources. F urther iTRAQ quantitative proteomics studies revealed that SPD-0090 affects galactose metabolism and iron ion transport system. Then RT-qPCR and intracellular galactose content assays showed that SPD-0090 affects galactose uptake, leads to reduced galactose utilization. In addition, in vitro biochemical assays showed that SPD-0090 is a hemin transporter and affects iron uptake. Notably the knockout of spd-0090 resulted in an enhanced infection ability of S.pneumonaie to Our study reveals that the dual function of SPD-0090 plays an important role in the virulence of S.pneumoniae.
HostingRepository	PRIDE
AnnounceDate	2022-06-01
AnnouncementXML	Submission_2022-06-01_06:15:07.822.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	cao linlin
SpeciesList	scientific name: Streptococcus pneumoniae D39; NCBI TaxID: 373153;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-05 04:42:14	ID requested
⏵ 1	2022-06-01 06:15:08	announced

Dataset with its publication pending

submitter keyword: Streptococcus pneumoniae, Iron transport protein, Galactose metabolism, Virulence, SPD-0090

Xuesong Sun
contact affiliation	Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou, China
contact email	tsunxs@jnu.edu.cn
lab head
cao linlin
contact affiliation	13797062336
contact email	479310394@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030183
     1. Label: PRIDE project
     2. Name: A Bifunctional SPD-0090 contributes virulence of Streptococcus pneumoniae by affecting iron transport and galactose metabolism