PXD030174-1

PXD030174 is an original dataset announced via ProteomeXchange.

Title	Co-IP of v-ATPase subcomplexes from MEFs under amino acid starvation and restimulation
Description	v-ATPase Vo and V1 domains were isolated via Co-IP from MEFs expressing HA-Atp6V1B2 and FLAG-Atp6VoaA3 using HA- and FLAG-tag antibodies. Treatment groups were full medium (control), amino acid starvation (60 min) and restimulation (30 min). Cells were grown in SILAC medium for 3 passages.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:43:34.138.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wilhelm Palm
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	6x(13)C; (4,4,5,5-(2)H4)-L-lysine; 6x(13)C labeled L-arginine; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-03 09:00:14	ID requested
⏵ 1	2024-10-07 13:43:34	announced

10.1038/S41467-022-32515-6;

submitter keyword: MEFs, Co-IP, SILAC, vATPase, Amino acid starvation

Wilhelm Palm
contact affiliation	German Cancer Research Center (DKFZ) Cell Signaling and Metabolism Group
contact email	w.palm@dkfz-heidelberg.de
lab head
Wilhelm Palm
contact affiliation	German Cancer Research Center (DKFZ)
contact email	w.palm@dkfz-heidelberg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD030174

PRIDE project URI

• PRIDE
  1. PXD030174
     1. Label: PRIDE project
     2. Name: Co-IP of v-ATPase subcomplexes from MEFs under amino acid starvation and restimulation