PXD030092-1

PXD030092 is an original dataset announced via ProteomeXchange.

Title	The proteome of irradiated glioma stem cells and of their extracellular vesicles
Description	Glioblastoma is a malignant brain tumor that is highly resistant to radiation and chemotherapy, where patients survive on average only 15 months after diagnosis. Furthering the understanding of mechanisms leading to radiation resistance of glioma is paramount to identify novel therapeutic targets. Previous studies have shown that glioma stem cells (GSCs) play an important role in promoting radiation resistance and disease recurrence. Herein we analyze the proteomic alterations occurring in patient-derived GSCs upon radiation treatment in order to identify molecular drivers of resistance. We show that proteome changes upon radiation accurately predict the resistance status of the cells, and that resistance to radiation does not correlate with glioma transcriptional subtypes. We further show that the radio-resistant cell line GSC-267 sheds microvesicles (MVs) enriched in the metabolic enzyme nicotinamide phosphoribosyltransferase (NAMPT).
HostingRepository	PRIDE
AnnounceDate	2023-04-06
AnnouncementXML	Submission_2023-04-06_14:44:21.212.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ElenaPanizza
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-02 02:05:00	ID requested
⏵ 1	2023-04-06 14:44:21	announced
2	2023-11-14 08:53:56	announced	2023-11-14: Updated project metadata.

submitter keyword: glioblastoma, glioma, radiation, glioma stem cells,human

Richard A.Cerione
contact affiliation	Department of Molecular Medicine, Cornell University, Ithaca, NY 14853 Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853
contact email	rac1@cornell.edu
lab head
ElenaPanizza
contact affiliation	Cornell University
contact email	elena.panizza84@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD030092

PRIDE project URI

• PRIDE
  1. PXD030092
     1. Label: PRIDE project
     2. Name: The proteome of irradiated glioma stem cells and of their extracellular vesicles