PXD030059-1

PXD030059 is an original dataset announced via ProteomeXchange.

Title	Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing (Frog) Embryo
Description	We provide a protocol to enable the mass spectrometry-based characterization of proteins in identified cell lineages in Xenopus laevis (frog) embryos. The experimental workflow leverages reproducible cell-fate maps and established technologies from developmental biology to identify, fluorescently label, track, and analyze cells and their progenies from this important biological model of vertebrate development. Proteins are identified and quantified via bottom-up proteomics. Microanalytical capillary electrophoresis or nanoflow liquid chromatography (nanoLC) are used to separate peptides, and electrospray ionization (ESI) high-resolution mass spectrometry (HRMS) are employed for detection.
HostingRepository	PRIDE
AnnounceDate	2022-02-23
AnnouncementXML	Submission_2022-02-23_11:29:16.378.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Nemes
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-30 07:50:23	ID requested
⏵ 1	2022-02-23 11:29:16	announced

Dataset with its publication pending

submitter keyword: Cell and developmental biology, neuroscience, neuron, proteomics, mass spectrometry, capillary electrophoresis, liquid chromatography, Xenopus laevis, frog

Peter Nemes
contact affiliation	Associate Professor, Department of Chemistry & Biochemistry, University of Maryland–College Park, MD
contact email	nemes@umd.edu
lab head
Peter Nemes
contact affiliation	University of Maryland, College Park
contact email	nemes@umd.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD030059

PRIDE project URI

• PRIDE
  1. PXD030059
     1. Label: PRIDE project
     2. Name: Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing (Frog) Embryo