PXD030044-1

PXD030044 is an original dataset announced via ProteomeXchange.

Title	Identification of BRCA1-associated protein 1 (BAP1)-associated proteins
Description	BRCA1-associated protein 1 (BAP1) is a multidomain deubiquitinase (DUB) with a ubiquitin carboxyl-terminal hydrolase (UCH) domain at its N-terminus. BAP1 mainly localizes in the nucleus and primarily functions as a transcriptional regulator in diverse cellular pathways, including cell proliferation, cell cycle progression, cell death and DNA repair. However, a comprehensive understanding of the mechanism by which the nucleocytoplasmic trafficking of BAP1 is regulated remains lacking. To identify protein factors that may be responsible for the nuclear import of BAP1, we used transiently expressed a N-terminally FLAG-tagged BAP1 in HEK293 freestyle (HEK293F) cells as a bait to pull down BAP1-interacting proteins for mass spectrometry-based proteomic analysis.
HostingRepository	PRIDE
AnnounceDate	2022-04-14
AnnouncementXML	Submission_2022-04-14_06:56:00.765.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tzu-Jing Yang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-29 08:45:19	ID requested
⏵ 1	2022-04-14 06:56:01	announced

Dataset with its publication pending

submitter keyword: Human, BRCA-1 associated protein 1, BAP1, tumor suppressor, deubiquitinase, DUB,

Shang-Te Danny Hsu
contact affiliation	1. Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan 2. Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan
contact email	sthsu@gate.sinica.edu.tw
lab head
Tzu-Jing Yang
contact affiliation	Institute of Biological Chemistry, Academia Sinica, Taiwan
contact email	jing0526@gate.sinica.edu.tw
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD030044

PRIDE project URI

• PRIDE
  1. PXD030044
     1. Label: PRIDE project
     2. Name: Identification of BRCA1-associated protein 1 (BAP1)-associated proteins